| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Purkinje cells are the sole output neurons of the cerebellar cortex and their dysfunction causes severe ataxia. We found that both Purkinje cells and granule cells could be effectively generated from human pluripotent stem (ES and iPS) cells by recapitulating the self-inducitive signaling microenvironments of the isthmic organizer. The cerebellar neuroepithelium (NE), formed in response to the combination of Fgf2 and insulin, differentiated into electrophysiologically functional Purkinje cells. The addition of Fgf19 and CXCL12 promotes the generation a continuous cerebellar plate NE and a three-layer cytoarchitecture similar to the embryonic cerebellum. Thus, human pluripotent stem cells-derived cerebellar progenitors exhibit substantial self-organizing potential for generating a polarized structure reminiscent of the early human cerebellum.
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