| Project/Area Number |
24570246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Eriko 山形大学, 基盤教育院, 准教授 (20337405)
NAKAUCHI Yuni 山形大学, 理学部, 助教 (60250908)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 体内受精 / 精子運動 / 機能進化 / 両生類 / 輸卵管 / 精子先体反応 / 精子運動開始 / ジェリー層 / 精子ー卵相互作用 |
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| Outline of Final Research Achievements |
SMIS, a key protein for internal fertilization of the newt, C. pyrrhogaster, localizes granules in the surface of egg jelly and induces sperm motility in female body. It acts in a different manner among amphibians and is hypothesized to contribute to the diversification of reproductive modes to evolve internal fertilization. To address this hypothesis, we examined ultrastructure of egg jelly and SMIS localization in C. ensicauda and P. waltl that undergo internal fertilization, R. arboreus that do arboreal fertilization, and B. japonicus that do external fertilization. Distributions of SMIS-localizing granules suggested changes in machinery of SMIS action adaptively to various factors including reproductive modes. We also found that C. pyrrhogaster SMIS gene was constructed by 3 exons and succeeded to establish a new method to identify SMIS homologous genes using RNAseq, which will be available to analyze the correlation of SMIS structure with its different action in future study.
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