| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we first showed that endogenous ascorbic acid (AsA) functioned as an antiviral chemical to TuMV in Brassica rapa and Arabidopsis thaliana. Next, it was shown that the AsA accumulation was induced by TuMV infection in the B. rapa resistant cultivar Aki-masari. This AsA accumulation was due to suppression of AsA oxidation by ascorbate peroxidase (APX) and ascorbate oxidase and activation of AsA recycling by dehydroascorbate reductase (DHAR). Because methyl jasmonate (MeJA) suppressed the APX activity and enhanced the DHAR activity; JA derivatives including JA-isoleucine, tuberonic acid and tuberonic acid glucoside were increased together with AsA, the induction of the AsA accumulation in Aki-masari appeared to be mediated by the JA-dependent signaling pathway suggesting that the AsA accumulation was induced as a defense reaction against virus.
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