| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Rose geranium produces citronellol, a monoterpene rosy-scent compound. In this study, I sequenced transcriptome of young leaves of the plant to establish an expressed sequence database. I explored candidates genes for monoterpene metabolism, and consequently, cloned three cDNAs encoding old yellow enzyme homologues. The recombinant enzymes exhibited double bond reductase activity producing citronellal from citral. It is quite interesting that rose geranium contains citral reducing enzymes possibly involved in monoterpene metabolism. The obtained cDNAs may be applicable to metabolic engineering to create plants with modified fragrance.
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