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Comprehensive analysis of wheat seed storage proteins associated with wheat allergy

Research Project

Project/Area Number 24580011
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Breeding science
Research InstitutionYokohama City University

Principal Investigator

KAWAURA Kanako  横浜市立大学, 木原生物学研究所, 准教授 (60381935)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsパンコムギ / 種子貯蔵タンパク質 / 小麦アレルギー / グリアジン / 遺伝子発現 / アレルギー
Outline of Final Research Achievements

In order to screen for hypoallergenic wheat, seed storage proteins extracted from 265 lines of various hexaploid wheat were evaluated by Western blot using three kinds of anti-peptide antibodies for epitope of wheat allergy.
Gliadins were selectively extracted from Chinese Spring wheat and its aneuploid lines. Two-dimensional electrophoresis profiles were compared among lines. Out of 70 gliadin spots, 52 spots could be determined their chromosome loci. Furthermore, gliadins which have the epitope of wheat allergy and are transcribed from chromosome 6D were specifically suppressed in the tetrasomic 2A lines, suggesting Chinese Spring wheat has a specific gliadin suppressor on chromosome 2A.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (9 results)

  • [Journal Article] Genome change in wheat observed through the structure and expression of α/β-gliadin genes2012

    • Author(s)
      Kawaura K, Wu J, Matsumoto T, Kanamori H, Katagiri S, Ogihara Y.
    • Journal Title

      Functional & Integrative Genomics

      Volume: 12(2) Issue: 2 Pages: 341-355

    • DOI

      10.1007/s10142-012-0269-0

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 3.形質転換体を用いたコムギ種子貯蔵タンパク質を制御する転写因子の機能解析2015

    • Author(s)
      神山春風、佐久間俊、荻原保成、川浦香奈子
    • Organizer
      日本育種学会第127回講演会
    • Place of Presentation
      玉川大学
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genome organization and expression of α/β-gliadin multigenes in common wheat2014

    • Author(s)
      Kanako Kawaura
    • Organizer
      Joint Symposium of Yokohama City University and Korea University
    • Place of Presentation
      Korea University (大韓民国ソウル市)
    • Year and Date
      2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] パンコムギ染色体異数体系統を用いたグリアジンタンパク質の同定2014

    • Author(s)
      三浦麻友子、川浦香奈子、中村真子、池田達哉、荻原保成
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Genomic structure and expression of a/b-gliadin genes in hexaploid wheat2013

    • Author(s)
      Kanako Kawaura, Jianzhong Wu, Takashi Matsumoto, Hiroyuki Kanamori, Satoshi Katagiri, Yasunari Ogihara
    • Organizer
      The 12th International Wheat Genetics Symposium
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Comparative analysis of wheat seed storage proteins in hexaploid wheat2013

    • Author(s)
      Mayuko Miura, Kanako Kawaura, Makiko Nakamura, Haruka Kouyama, Yasunari Ogihara
    • Organizer
      The 12th International Wheat Genetics Symposium
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Molecular characterization of the a/b-gliadin multigene family in hexaploid wheat2013

    • Author(s)
      Satoshi Noma, Katsuyuki Hayakawa, Kanako Kawaura, Yasunari Ogihara
    • Organizer
      The 12th International Wheat Genetics Symposium
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 1. Genomic analysis of the expressed α/β-gliadin gene region in hexaploid wheat.2012

    • Author(s)
      K. Kawaura, J. Wu, T. Matsumoto, H. Kanamori, S. Katagiri, Y. Ogihara.
    • Organizer
      11th International Gluten Workshop
    • Place of Presentation
      中華民国 北京市
    • Related Report
      2012 Research-status Report
  • [Presentation] 2. Profiling of gliadins from common wheat by using two-dimensional gel electrophoresis2012

    • Author(s)
      M. Miura, K. Kawaura, M. Nakamura, H. Kouyama, Y. Ogihara
    • Organizer
      11th International Gluten Workshop
    • Place of Presentation
      中華民国 北京市
    • Related Report
      2012 Research-status Report
  • [Presentation] 二次元電気泳動を用いたパンコムギのグリアジンに比較解析2012

    • Author(s)
      三浦麻友子、川浦香奈子、神山春風、中村真子、荻原保成
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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