| Project/Area Number |
24580025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Crop science/Weed science
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| Research Institution | Kagawa University |
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Principal Investigator |
AGARIE Sakae 香川大学, 農学部, 教授 (50304879)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | アイスプラント / 遺伝子発現 / 塩生植物 / 耐塩性 / トライコーム / ブラッダー細胞 |
|---|
| Outline of Final Research Achievements |
The common ice plant Mesembryanthemum crystallinum L., a halophyte develops epidermal bladder cells (EBCs) on the aerial surfaces for the sequestration of excess salt from metabolically active tissues. To elucidate the molecular determinants governing EBC development, we examined the relative steady-state mRNA abundance of orthologs of cotton fiber-related genes and Arabidopsis trichome-related genes in wild-type plants and an mutant lacking EBCs. We also isolated genes by cDNA-based suppressive-subtractive hybridization (SSH) PCR. Among them, a putative GLABRA2 and a MYB transcript factor homolog were preferentially expressed in wild-type plants, whereas a putative TRIPTYCHON and CAPRICE-like gene were preferentially expressed in the EBC mutant. Two of SSH-derived clones (WM10 and WM28) were preferentially expressed in wild-type plants, whereas MW3, MW21 and MW31 were preferentially expressed in the mutant. The WM10 and WM28 showed no similarity to any genes previously reported.
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