| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Production methods for periclinal chimeric plants on chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri) were examined. Leaf explants of wild-type plants and transgenic plants having a fluorescent protein gene were placed on medium close to each other, and detached just after they partially combined. After 1 week culture on medium with an antibiotics for selection, mosaic calli consisted of transgenic cells and wild-type cells were formed on the edge of explants. Regenerated shoots (chrysanthemum 996, torenia 357) were analyzed by observation of fluorescence to detect chimeric structure. No chimeric plants was obtained on torenia, but a single sectorial- chimeric shoot was obtained in chrysanthemum. Chrysanthemum plants of L1 periclinal chimera and L3 periclinal chimera were obtained from the sectorial-chimeric shoot after repeated axillary bud elongation.
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