| Project/Area Number |
24580058
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Saga University (2013-2014)
独立行政法人農業・食品産業技術総合研究機構 (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Satoshi 独立行政法人農業・食品産業技術総合研究機構, 野菜茶業研究所, 研究員 (20414675)
HONDA Ichiro 前橋工科大学, 工学部, 教授 (00241852)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | カンキツ / ウンシュウミカン / 単為結果性 / ジベレリン / GA20酸化酵素遺伝子 / GA2酸化酵素遺伝子 / マイクロアレイ / 植物ホルモン / 果樹園芸 / 植物遺伝資源 / ゲノム解析 |
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| Outline of Final Research Achievements |
We investigated the degree of parthenocarpy of four citrus cultivars, Satsuma mandarin, Kiyomi, Hyuganatsu, and clementine. Especially, Satsuma mandarin had higher rate of development of ovaries at the early stage besides strong parthenocarpy. Microarray analysis suggested that there was difference in gene expression of gibberellin (GA)-biosynthetic pathway genes between parthenocarpy and non-parthenocarpy cultivars. Therefore, GA 20-, GA 2-, and GA 3-oxidase genes of Satsuma mandarin were isolated and subjected to functional analysis, resulting in the identification of enzymatic genes that have an activity of growth promotion or retardation.
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