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Intensive mapping of the male fertility restoring gene locus and identification of cytoplasm type in bunching onion

Research Project

Project/Area Number 24580060
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

YAMASHITA Ken-ichiro  独立行政法人農業・食品産業技術総合研究機構, 野菜茶業研究所 野菜育種・ゲノム研究領域, 主任研究員 (80414671)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsネギ / 細胞質雄性不稔性 / 稔性回復遺伝子 / CMS / DNAマーカー / CMS / DNAマーカー
Outline of Final Research Achievements

Linkage analysis of the fertility restoring gene locus, Ms3, was conducted in F2 population between bunching onion ‘Kinryo’ and ‘Pekin’ using SSR markers etc. The Ms3 locus was located on the chromosome 3, and the closely linked marker positioned at 6.3cM from the Ms3 locus. Pollen fertility restoration was investigated in progeny produced from crossings between CMS plants of six commercial F1 cultivars and male fertile ‘Kinryo’ or ‘Pekin’ with Ms3 gene heterozygously. As the results, segregation ratios of male fertile to male sterile plants fitted to probability of 1 to 1 in the two progeny, which confirms that the inheritance mode of CMS from the two F1 cultivars is identical to those of‘Kinryo’ and ‘Pekin’.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2014 2013

All Presentation (2 results)

  • [Presentation] ネギの細胞質雄性不稔性に対する稔性回復遺伝子Ms3と連鎖するDNAマーカーの開発2014

    • Author(s)
      山下謙一郎
    • Organizer
      園芸学会平成26年度秋季大会
    • Place of Presentation
      佐賀大学本庄キャンパス
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネギ類における雄性不稔性の遺伝様式解明および育種利用2013

    • Author(s)
      山下謙一郎
    • Organizer
      平成25年度野菜茶業課題別研究会
    • Place of Presentation
      愛知県産業労働センター(ウインクあいち)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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