| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Outline of Final Research Achievements |
Mutant selection is an important breeding technology, but the detail of mutation has not been understood. We evaluated whole genome sequences of five satsuma mandarin lines in order to identify the mutation among them. The sequence analysis identified more than 40,000 SNPs and 6,000 indels specific for individual mutant lines. Most of them were found in non-genic region, and observed mutation frequency suggested that replication error of DNA polymerase is a major cause of mutation. DNA markers developed from SNPs or indels located in genic region revealed their stable maintenance in posterior mutant lines, or in nucellar seedlings.These observations suggested frequent occurrence of mutation, but a few of them would substitute normal cell in a tissue, then maintained stably. No obvious increase of mutation detected on nucellar line also suggested that a random selection of existing mutant cells would be the major source of mutant in nucellar seedling.
|
