Endogenization process of non-retroviral RNA virus elements into plant genomes and their pathological significance

• Project/Area Number: 24580064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Okayama University
• Principal Investigator: KONDO Hideki 岡山大学, 資源植物科学研究所, 准教授 (40263628)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Nobuhiro 岡山大学, 資源生物科学研究所, 教授 (70206514)
• Research Collaborator: MARUYAMA Kazuyuki 岡山大学, 資源植物科学研究所, 技術職員 (20379811)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 非レトロRNAウイルス / ゲノム / 感染記録 / うどんこ病菌 / 化石配列 / ダラースポット病菌 / 系統進化 / モノネガウイルス / ウイルス化石 / ベニウイルス

Outline of Final Research Achievements

Endogenous non-retroviral RNA virus-like sequences (NRVSs) have been discovered in the genome of the vertebrates and other eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms. In this study, several novel NRVSs from the genome of the plants, insects and fungi were discovered. The presence of benyvirus-like sequences in the chickpea chromosomes is a second example of plant NRVSs related to positive-sense (+)ssRNA viruses. Benyvirus-related sequences were also found in the chromosomes of blood-sucking insect. In addition, the first (-)RNA virus infection in fungus was evidenced based on a discovery of mononegavirus L-like sequences in the genome of the powdery mildew fungus. Our findings may provide novel insights into the origin and evolution of ssRNA viruses. The possible endogenization process of these NRVSs into the genome and its biological significance on the host were discussed.

Research Products

• [Journal Article] Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus. (2014)
  • Author(s): Kondo, H., Maruyama, K., Chiba, S., Andika, I. B., Tamada, T. and Suzuki, N.
  • Journal Title: Virology Volume: 452-453 Pages: 166-174
  • DOI: 10.1016/j.virol.2014.01.007
  • Peer Reviewed
• [Journal Article] Discovery of negative-strand RNA virus infection in fungi. (2014)
  • Author(s): Kondo, H., Chiba, S. and Suzuki, N.
  • Journal Title: Proc. Symp. 9th Int. Working Group on Plant Viruses with Fungal Vectors Volume: 1 Pages: 55-57
• [Journal Article] Characterazation of burdock mottle virus, a novel member of the genus Benyvirus, and the presence of benyvirus-related sequences in the plant and insect genomes. (2013)
  • Author(s): Kondo, H. , Hirano, S., Chiba, S., Andika, I.B., Hirai, M., Maeda, T. and Tamada, T.
  • Journal Title: Virus Research Volume: 177 Issue: 1 Pages: 75-86
  • DOI: 10.1016/j.virusres.2013.07.015
  • Peer Reviewed
• [Journal Article] Evidence for the infection of fungi by of negative-strand RNA viruses (Essential Collection, Editors’ Selection 2013). (2013)
  • Author(s): Kondo, H., Chiba, S., Toyoda, K. and Suzuki, N.
  • Journal Title: Virology Volume: 435 Issue: 2 Pages: 201-209
  • DOI: 10.1016/j.virol.2012.10.002
  • Peer Reviewed
• [Journal Article] 分節型ゲノムを持つラブドウイルス (2013)
  • Author(s): 近藤秀樹
  • Journal Title: ウイルス Volume: 63 Pages: 143-154
  • NAID: 130004713420
• [Journal Article] Nyamiviridae: Proposal for a new family in the order Mononegavirales (2013)
  • Author(s): Kuhn, J.H., Bekal, S., Caì, Y., Clawson, A.N., Domier, L.L., Herrel, M., Jahrling, P.B., Kondo, H., Lambert, K.N., Mihindukulasuriya, K.A., Nowotny, N., Radoshitzky, S.R., Schneider, U., Staeheli, P., Suzuki, N., Tesh, R.B., Wang, D, Wang, L-F., and Dietzgen, R.G.
  • Journal Title: Archives of Virology Volume: 158 Issue: 10 Pages: 2209-2226
  • DOI: 10.1007/s00705-013-1674-y
  • Peer Reviewed
• [Journal Article] Orchid fleck virus structural protein, N and P, form intranuclear viroplasm-like structures in the absence of viral infection (2013)
  • Author(s): Kondo, H., Chiba, S., Andika, I.B., Maruyama, K., Tamada T. and Suzuki, N.
  • Journal Title: Journal of Virology Volume: 87 Issue: 13 Pages: 7423-7434
  • DOI: 10.1128/jvi.00270-13
  • Peer Reviewed
• [Journal Article] Viruses of the white root rot fungus, Rosellinia necatrix. (2013)
  • Author(s): Kondo, H., Kanematsu, S. and Suzuki, N.
  • Journal Title: Advances in Virus Research Volume: 86 Pages: 177-214
  • DOI: 10.1016/b978-0-12-394315-6.00007-6
  • ISBN: 9780123943156
  • Peer Reviewed
• [Journal Article] Create 1 new species in the genus Benyvirus and assign the genus to the new family Benyviridae. (2013)
  • Author(s): Gilmer, D., Ratti, C., Tamada, T., Andika, I.B. and Kondo, H.
  • Journal Title: ICTV Taxonomy Proposal (TaxoProp) Volume: Plant Pages: 9-9
• [Journal Article] Effects of defective-interfering RNA on symptom induction and replication of a novel partitivirus from a phytopathogenic fungus Rosellinia necatrix. (2013)
  • Author(s): Chiba, S., Lin, Y-H., *Kondo, H., Kanematsu, S., and Suzuki, N.
  • Journal Title: Journal of Virology Volume: 87 Issue: 4 Pages: 2330-2341
  • DOI: 10.1128/jvi.02835-12
  • Peer Reviewed
• [Journal Article] The cysteine-rich proteins of beet necrotic yellow vein virus and tobacco rattle virus contribute to efficient suppression of silencing in roots (2012)
  • Author(s): Andika, I.B., *Kondo, H., Nishiguchi, M. and Tamada, T.
  • Journal Title: Journal of General Virology Volume: 93 Issue: 8 Pages: 1841-1850
  • DOI: 10.1099/vir.0.043513-0
  • Peer Reviewed
• [Presentation] Deep sequencing解析により明らかになったBotrytis tulipae の９種ウイルスによる混合感染 (2015)
  • Author(s): 近藤秀樹・久野 昌・林 諭昕・千葉壮太郎・鈴木信弘
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] 近藤 秀樹・千葉 壮太郎・鈴木 信弘. 菌類に感染するマイナス鎖RNAウイルス (2014)
  • Author(s): 近藤 秀樹・千葉 壮太郎・鈴木 信弘
  • Organizer: 29回中国四国ウイルス研究会
  • Place of Presentation: 山口
  • Year and Date: 2014-06-28 – 2014-06-29
• [Presentation] 2分節マイナス鎖RNAウイルスであるランえそ斑紋ウイルス(OFV)のmRNAおよびleader RNAの特性 (2014)
  • Author(s): 近藤秀樹・丸山和之・千葉壮太郎・鈴木信弘
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] 植物ゲノム上のRNA感染記録から紐解くウイルス-宿主相互作用 (2013)
  • Author(s): 近藤秀樹
  • Organizer: 第30回資源植物科学シンポジウム及び第6回植物ストレス科学研究シンポジウム
  • Place of Presentation: 倉敷市芸文館
  • Invited
• [Presentation] Discovery of negative-strand RNA virus infection in fungi (2013)
  • Author(s): Kondo, H., Chiba, S. and Suzuki, N.
  • Organizer: 9th Int. Working Group on Plant Viruses with Fungal Vectors
  • Place of Presentation: Obihiro
• [Presentation] 植物および昆虫の核ゲノム上に見いだされたベニウイルス様配列 (2013)
  • Author(s): 近藤秀樹・千葉壮太郎・鈴木信弘
  • Organizer: 平成25年度日本植物病理学会関西部会
  • Place of Presentation: 岡山大学
• [Presentation] 新規ベニウイルス・burdock mottle virus(BdMoV)の配列解析とベニウイルス類似配列の植物・昆虫ゲノム上での発見 (2013)
  • Author(s): 近藤秀樹・千葉壮太郞・Ida Bagus Andika・鈴木信弘・玉田哲男
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] マイナス鎖RNAウイルスの菌類への感染の可能性 (2013)
  • Author(s): 近藤秀樹・千葉壮太郎・豊田和弘・鈴木信弘
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学
• [Presentation] マイナス鎖RNAウイルスの菌類への感染の可能性 (2012)
  • Author(s): 近藤秀樹・千葉壮太郎・鈴木信弘
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Evolutionary history of the family Partitiviridae: insight from the viewpoint of paleovirology. (2012)
  • Author(s): Chiba, S., Kondo, H., Kanematsu, S., Suzuki, N.
  • Organizer: The American Society for Virology, 31st Annual Meeting (ASV)
  • Place of Presentation: Wisconsin, USA
• [Book] Detection and analysis for non-retroviral RNA virus-like elements in plant, fungal and insect genomes. in Plant Virology Protocols (Methods in Molecular Biology 1236, Springer Protocol): New approaches to detect viruses and host responses. eds, Uyeda, I and Masuta C. (2015)
  • Author(s): Kondo, H., Chiba, S. and Suzuki, N.
  • Publisher: Humana Press Inc.
• [Remarks] 岡山大学資源植物科学研究所 植物・微生物相互作用グループ
  • URL: http://www.rib.okayama-u.ac.jp/pmi/index-j.html
• [Remarks] 岡山大学資源植物科学研究所 植物・微生物相互作用グループ
  • URL: http://www.rib.okayama-u.ac.jp/pmi/index-j.html