Project/Area Number |
24580066
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant pathology
|
Research Institution | Kochi University |
Principal Investigator |
KIBA Akinori 高知大学, 教育研究部総合科学系, 教授 (50343314)
|
Co-Investigator(Kenkyū-buntansha) |
HIKICHI Yasufumi 高知大学, 教育研究部総合科学系, 教授 (70291507)
OHNISHI Kouhei 高知大学, 教育研究部総合科学系, 教授 (50211800)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 植物 / 免疫 / フォスファチジン酸 / 耐病性 / 植物免疫 / プライミング / 植物病原細菌 / 病害防除 / タバコ植物 / 免疫応答 / 耐病性植物 / 植物細菌病 / リン脂質 |
Outline of Final Research Achievements |
DS1 plants that showed accelerated defense responses in response to compatible Ralsto nia solanacearum infection. We analyzed activation mechanisms of defense responses in DS1 plants.DS1 plants showed hyper induction of hypersensitive response and reactive oxygen species generation. Acceleration of HR and ROS was closely associated with enhanced phosphatidic acid contents. We created DS1-knockdown transgenic tobacco, that showed resistance against R. solanacearum. We also created DS1-knockdown transgenic tomato. In addition, we screened DS1-deficient mutant of Arabisopusis. Therefore, we will analyze immune response using DS1-knockdown tobacco and tomato, and DS-feficient Arabidopsis. In addition, we successfully isolated promoter region of DS1 gene, and we started promoter analysis of DS1 gene.
|