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Analysis of conferred disease resistance by artificial control of phosphatidc acid metabolism in plants

Research Project

Project/Area Number 24580066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant pathology
Research InstitutionKochi University

Principal Investigator

KIBA Akinori  高知大学, 教育研究部総合科学系, 教授 (50343314)

Co-Investigator(Kenkyū-buntansha) HIKICHI Yasufumi  高知大学, 教育研究部総合科学系, 教授 (70291507)
OHNISHI Kouhei  高知大学, 教育研究部総合科学系, 教授 (50211800)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords植物 / 免疫 / フォスファチジン酸 / 耐病性 / 植物免疫 / プライミング / 植物病原細菌 / 病害防除 / タバコ植物 / 免疫応答 / 耐病性植物 / 植物細菌病 / リン脂質
Outline of Final Research Achievements

DS1 plants that showed accelerated defense responses in response to compatible Ralsto nia solanacearum infection. We analyzed activation mechanisms of defense responses in DS1 plants.DS1 plants showed hyper induction of hypersensitive response and reactive oxygen species generation. Acceleration of HR and ROS was closely associated with enhanced phosphatidic acid contents. We created DS1-knockdown transgenic tobacco, that showed resistance against R. solanacearum. We also created DS1-knockdown transgenic tomato. In addition, we screened DS1-deficient mutant of Arabisopusis. Therefore, we will analyze immune response using DS1-knockdown tobacco and tomato, and DS-feficient Arabidopsis. In addition, we successfully isolated promoter region of DS1 gene, and we started promoter analysis of DS1 gene.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (30 results)

All 2015 2014 2013 2012

All Journal Article (18 results) (of which Peer Reviewed: 18 results,  Open Access: 2 results) Presentation (12 results)

  • [Journal Article] Multiplex polymerase chain reaction discriminates which eggplant isolates of Corynespora cassiicola are virulent to sweet pepper2015

    • Author(s)
      Yoshifumi Shimomoto, Akinori Kiba and Yasufumi Hikichi.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 81 Issue: 3 Pages: 226-231

    • DOI

      10.1007/s10327-015-0584-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Implication of limited iron acquisition of Pseudomonas cichorii strain SPC9018 in reduction of its virulence on eggplant.2015

    • Author(s)
      Ullah Md Wali, Risa Maenaka, Yuka Mori, Daisei Ueno, Kenji Kai, Kouhei Ohnishi, Akinori Kiba, Hideo Hayashi, and Yasufumi Hikichi
    • Journal Title

      Journal of General Plant Pathology

      Volume: 81 Pages: 136-141

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ralfuranones J&L, quorum sensing-regulated metabolites from Ralstonia solanacearum OE1-1.2015

    • Author(s)
      Kenji Kai, Hideyuki Ohnishi, Yuka Mori, Akinori Kiba, Kouhei Ohnishi, and Yasufumi Hikichi
    • Journal Title

      ChemBioChem

      Volume: 15 Pages: 2590-2597

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular chaperons and co-chaperons, Hsp90, RAR1, and SGT1 negatively regulate bacterial wilt disease caused by Ralstonia solanacearum in Nicotiana benthamiana.2015

    • Author(s)
      Makoto Ito, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba
    • Journal Title

      Plant signaling & behavior

      Volume: 9 Issue: 6 Pages: 1-5

    • DOI

      10.4161/15592316.2014.970410

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of the mutations in Pepper mild mottle virus overcoming tomato tm-1-mediated resistance.2014

    • Author(s)
      Hiroyuki Mizumoto, Yukino Morikawa, Kazukazuhiro Ishibashi, Kentaro Kimura, Kouhei Matsumoto, Masayuki Tokunaga, Akinori Kiba, Masayuki Ishikawa, Tetsuro Okuno, and Yasufumi Hikichi.
    • Journal Title

      Molecular Plant Pathology

      Volume: 15 Pages: 479-487

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SEC14 phospholipid transfer protein involve in lipid signaling-mediated plant immune response in Nicotiana benthamiana.2014

    • Author(s)
      Akinori Kiba, Ivan Galis, Yuko Hojo, Kouhei Ohnishi, Hirofumi Yoshioka, Yashufumi Hikichi.
    • Journal Title

      PLOS ONE

      Volume: 9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Silencing of DS2 aminoacylase-like genes confirms basal resistance to Phytophthora infestans in Nicotiana benthamiana.2014

    • Author(s)
      Nakano, M., Nishihara, M., Yoshioka, H., Ohnishi, K., Hikichi, Y., and Kiba, A.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfire disease in Nicotiana benthamiana.2014

    • Author(s)
      Ito, M., Takahashi, H., Sawasaki, T., Ohnishi, K., Hikichi, Y., and Kiba, A.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 9

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heat shock protein 70 is required for tabtoxinine-β-lactam-induced cell death in Nicotiana benthamiana.2014

    • Author(s)
      Ito, M., Yamamotob, Y., Kim, C-S., Ohnishi, K., Hikichi, Y., and Kiba, A.
    • Journal Title

      Journal of Plant Physiology

      Volume: 171 Pages: 173-178

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Suppression of DS1 phosphatidic acid phosphatase confirms resistance to Ralstonia solanacearum in Nicotiana benthamiana.2013

    • Author(s)
      Nakano, M., Nishihara, M., Yoshioka, H., Takahashi, H., Sawasaki, T., Ohnishi, K., Hikichi, Y., and Kiba, A.
    • Journal Title

      Plos One

      Volume: 8

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A translationally controlled tumor protein negatively regulates the hypersensitive response in Nicotiana benthamiana.2013

    • Author(s)
      Gupta, M., Yoshioka, H., Ohnishi, K., Mizumoto, H., Hikichi, Y., and Kiba, A
    • Journal Title

      Plant Cell & Physiology

      Volume: 54 Pages: 1403-1414

    • NAID

      10031195192

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants.2012

    • Author(s)
      Daisuke Komori, Masahiro Nishihara, Akiko Takahashi, Meenu Gupta, Hirofumi Yoshioka, Hiroyuki Mizumoto, Kouhei Ohnishi, Yusufumi Hikichi, Akinori Kiba.
    • Journal Title

      Plant Biotechnology

      Volume: 29 Pages: 293-300

    • NAID

      10030804810

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity.2012

    • Author(s)
      Akinori Kiba, Masahito Nakano, Patrick Vincent-Pope, Hirotaka Takahashi, Tatsuya Sawasaki, Yaeta Endo, Kouhei Ohnishi, Hirofumi Yoshioka, Yasufumi Hikichi.
    • Journal Title

      Journal of Plant Physiology

      Volume: 169 Pages: 1017-1022

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional differentiation in the LRR domains of closely related plant virus resistance proteins that recognize common Avr proteins.2012

    • Author(s)
      Ken-Taro Sekine, Reiko Tomita, Shigeharu Takeuchi, Go Atsumi, Hiromasa Saitoh, Hiroyuki Mizumoto, Akinori Kiba, Naoto Yamaoka, Masamichi Nishiguchi, Yasufumi Hikichi and Kappei Kobayashi.
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 25 Pages: 1219-1229

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Amino acids in Tobamovirus coat protein controlling pepper L1a gene-mediated resistance.2012

    • Author(s)
      Hiroyuki Mizumoto, Ikumi Nakamura, Yoshifumi Shimomoto, Hiromasa Sawada, Reiko Tomita, Ken-taro Sekine, Akinori Kiba, Masamichi Nishiguchi, Kappei Kobayashi and Yasufumi Hikichi.
    • Journal Title

      Molecular Plant Pathology

      Volume: 13 Pages: 915-922

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 葯培養によるピーマン黒枯病抵抗性育種母本の育成2012

    • Author(s)
      下元祥史・澤田博正・岡田昌久・森田泰彰・水本祐之・木場章範・曵地康史.
    • Journal Title

      日本植物病理学会報

      Volume: 78 Pages: 85-91

    • NAID

      10030312036

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] mplication of hrpW in virulence of Pseudomonas cichorii.2012

    • Author(s)
      Shigeru Kajihara, Hiroshi Hojo, Makoto Koyanagi, Masayuki Tanaka, Hiroyuki Mizumoto, Kouhei Ohnishi, Akinori. Kiba and Yasufumi Hikichi.
    • Journal Title

      Plant Pathology

      Volume: 61 Pages: 355-363

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gentian lipid transfer protein homolog with antimicrobial properties confers resistance to Botrytis cinerea in transgenic tobacco.2012

    • Author(s)
      Akinori Kiba, Takashi Nakatsuka, Saburo Yamamura, Masahiro Nishihara.
    • Journal Title

      Plant Biotechnology

      Volume: 29 Pages: 95-101

    • NAID

      10030561589

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] DS1-フォスファチジン酸フォスファターゼ抑制植物における植物免疫活性化機構の解析2014

    • Author(s)
      木場章範、中野真人、吉岡博文、大西浩平、曵地康史
    • Organizer
      日本植物脂質科学研究会
    • Place of Presentation
      静岡県、静岡市、静岡市産学交流センター
    • Year and Date
      2014-11-28 – 2014-11-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Phosphatidic acid participates in regulation of plant immune responses in Nicotiana benthamiana.2014

    • Author(s)
      Kiba A, Nakano M, Ohnishi K, Hikichi Y
    • Organizer
      3rd Korea-Japan Joint Symposium on Plant Pathology
    • Place of Presentation
      大韓民国、釜山、Pukyoung National University
    • Year and Date
      2014-10-23 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] フォスファチジン酸生合成と植物免疫2014

    • Author(s)
      木場章範、大西浩平、曵地康史
    • Organizer
      日本細菌学会
    • Place of Presentation
      タワーホール船堀(東京)
    • Related Report
      2013 Research-status Report
  • [Presentation] Phosphatidic acid participates in regulation of plant immune responses.2013

    • Author(s)
      Akinori KIba
    • Organizer
      Asian symposium of plant lipids
    • Place of Presentation
      金大中コンベンションセンター(大韓民国・光州)
    • Related Report
      2013 Research-status Report
  • [Presentation] 植物免疫応答におけるフォスファチジン酸生合成系の役割2013

    • Author(s)
      木場章範、夏目瑞紀、与那原由貴、中野真人、吉岡博文、大西浩平、曵地康史
    • Organizer
      日本植物脂質研究会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Cyclic AMP is involved in tabtoxinine-beta-lactam-induced cell death2013

    • Author(s)
      M. Itoh, Y. Yamamoto, C.-S. Kim, K. Ohnishi, H. Mizumoto, Y. Hikichi, A. Kiba
    • Organizer
      第54回 日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] cAMPはtabtoxinine-β-lactamにより誘導される細胞死に関与する2013

    • Author(s)
      伊藤 慎・山本 優・金 哲史・大西浩平・水本裕之・曵地康史・木場章範
    • Organizer
      平成25年度 日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 夏目瑞紀、水本祐之、大西浩平、曵地康史、木場章範2013

    • Author(s)
      Nicotiana benthamiana由来のジアシルグリセロールキナーゼ(NbDGK7-1)の植物免疫への関与
    • Organizer
      平成25年度 日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Meenu G, Yoshioka H, Ohnishi K, Mizumoto H, Hikichi Y, Kiba A2013

    • Author(s)
      Involvement of the translationall controlled tumore protein gene in negative regulation of hypersensitive response in Nicotiana benthamiana
    • Organizer
      平成25年度 日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Research-status Report
  • [Presentation] HSP70 regulates Tabtoxinine-beta-lactam-induced cell death in Nicotiana benthamiana2012

    • Author(s)
      M. Itoh, Y. Yamamoto, C.-S. Kim, K. Ohnishi, H. Mizumoto, Y. Hikichi, A. Kiba
    • Organizer
      XV International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      京都国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 木場章範、中野真人、吉岡博文、水本祐之、大西浩平、曵地康史2012

    • Author(s)
      フォスファチジン酸を介した植物の免疫応答
    • Organizer
      第25回植物脂質シンポジウム
    • Place of Presentation
      甲南大学(兵庫県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 夏目瑞紀、水本祐之、大西浩平、曵地康史、木場章範2012

    • Author(s)
      植物免疫におけるジアシルグリセロールキナーゼの役割
    • Organizer
      第25回植物脂質シンポジウム
    • Place of Presentation
      甲南大学(兵庫県)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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