Molecular mechanisms of synergism between plant viruses

• Project/Area Number: 24580067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyushu University
• Principal Investigator: TAKESHITA Minoru 九州大学, (連合)農学研究科(研究院), 助教 (00304767)
• Co-Investigator(Kenkyū-buntansha): 古屋 成人 九州大学 (10211533) ; 土屋 健一 九州大学 (40150510)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: CMV / TuMV / synergism / サリチル酸 / 2bタンパク質 / 局部干渉 / 混合感染 / 2b遺伝子 / Cucumber mosaic virus / ２ｂ / HC-Pro / Local interference / Turnip mosaic virus / local interference

Outline of Final Research Achievements

Spatial interactions between Cucumber mosaic virus (CMV) and Turnip mosaic virus (TuMV) were analyzed in Nicotiana benthamiana for better understand of the molecular mechanisms associated with local interference which can be observed before establishment of plant viral synergism. CMV and TuMV vectors expressing fluorescent protein DsRed2 (Ds) and E-GFP (EG) were used for inoculation onto N. benthamiana, respectively. A1 vector (A1) has a functional 2b while H1 vector (H1) is 2b-deficient. Curiously, a combination of H1Ds and TuEG compromised local interference in N. benthamiana nahG transgenic line. The results suggested that the SA-mediated host resistance and the 2b protein of CMV are involved in local interference.

Research Products

• [Journal Article] A single amino acid at N-terminal region of the 2b protein of cucumber mosaic virus strain m1 has a pivotal role in virus attenuation. (2015)
  • Author(s): Maneechoat, P., Takeshita, M., Uenoyama, M., Nakatsukasa, M., Kuroda, A., Furuya, N., Tsuchiya, K.
  • Journal Title: Virus Research Volume: 197 Pages: 67-74
  • DOI: 10.1016/j.virusres.2014.12.020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Induction of antiviral responses by acibenzolar-S-methyl against Cucurbit chlorotic yellows virus in melon (2013)
  • Author(s): Minoru Takeshita, Mitsuru Okuda, Shiori Okuda, Ayaka Hyodo, Kaori Hamano, Naruto Furuya, Kenichi Tsuchiya
  • Journal Title: Phytopathology Volume: 103 Issue: 9 Pages: 960-965
  • DOI: 10.1094/phyto-08-12-0188-r
  • Peer Reviewed
• [Presentation] 混合感染における植物ウイルス間の局部干渉に関する解析 (2015)
  • Author(s): 小田裕太・竹下 稔・吉岡博文・古屋成人・土屋健一
  • Organizer: 日本植物病理学会
  • Place of Presentation: 明治大学
  • Year and Date: 2015-03-30
• [Presentation] Biological and molecular characterization of a mild strain of Cucumber mosaic virus (2013)
  • Author(s): Phoowanarth Maneechoat, Atsuko Kuroda, Misa Uenoyama, Maki Nakatsukasa, Minoru Takeshita, Naruto Furuya, Tsuchiya Kenichi
  • Organizer: The 10th International Joint Symposium among Japan, Korea and China
  • Place of Presentation: 宮崎サンホテルフェニックス
• [Presentation] 2種ウイルスのシナジズムと局部干渉 (2013)
  • Author(s): 竹下 稔・小泉恵美子・野口眞季子・古屋成人・土屋健一
  • Organizer: 第11回植物ウイルス病研究会
  • Place of Presentation: 岐阜大学
  • Invited
• [Presentation] アシベンゾラル S メチル(ASM)によるキュウリモザイクウイルス(CMV)の感染低減に関する解析 (2013)
  • Author(s): 兵頭紋佳・竹下 稔・泊 賢吾・奥田 充・増田 税・古屋成人・土屋健一
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学
• [Book] 第11回植物ウイルス病研究会レポート (2013)
  • Author(s): 竹下 稔・小泉恵美子・野口眞季子・古屋成人・土屋健一
  • Total Pages: 10
  • Publisher: 2種ウイルスのシナジズムと局部干渉