| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Outline of Final Research Achievements |
To study somatic homologous recombination (HR) in Pyricularia oryzae, we created an HR detection system, using which we showed that somatic HR occurs at high levels in P. oryzae after treatment with several chemical agents. Somatic HR was also seen to be involved in the instability and diversification of the avirulence gene Avr-Pita in P. oryzae. We found that DNA double-strand breaks (DSBs) generated by the endonuclease I-SceI increased the frequency of ectopic HR in the genome of P. oryzae. To generate DSBs at a specific site in the desired locus within the P. oryzae genome, we designed and engineered nucleases called Platinum-Fungal TALENs. To summarize, genetic mutations caused by somatic HR in P. oryzae are responsible for the diversification of its virulence.
|
