| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Solanum integrifolium and Solanum torvum are useful rootstocks to avoid bacterial wilt of eggplants. In order to know the mechanism of host range expansion in R. solanacearum, we compared type III effector repertoires among R. solanacearum strains with different host ranges. Three effector genes, namely, rip10, rip36 and popP1, were not detected in the group III or group IV strains, which are virulent on S. integrifolium. Mutation analysis revealed that Rip36 is specifically recognized as an avirulence factor by both S. integrifolium and S. torvum. The rip36 mutant of the group II strain, which is avirulent on S. torvum, effectively cause disease in S. integrifolium. However, the rip36 mutant could not cause disease in S. torvum despite the increased multiplication, indicating that another avirulence factor for S. torvum exists in this strain. These findings suggest that the group IV strains are generated from the group III strains by as yet unidentified mutations.
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