| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The early male-killing strain of H. magnanima (Shizuoka strain) was collected in a tea field in Shizuoka, 2009. Purpose of this study was to identify the early male-killing agent. By running PCR with Wolbachia and Spiroplasma primers, the Shizuoka strain was infected with both bacteria. The strain which infected only Spiroplasma was produced by injecting larval hemolymph obtained from the Shizuoka strain to the Bacteria free strain which was established by continuously administering an antibiotic to the Shizuoka strain. No male was obtained from the Spiroplasma-infected strain, suggesting that the causative agent of early male-killing is Spiroplasma.
The generation changes in the prevalence of RNA virus (the late male-killing agent) and Spiroplasma in a natural population of H. magnanima were changed from 4.3 to 12.9% and 0.9 to 15.1%, respectively. Occurrence of H. magnanima infected with both RNA virus and Spiroplasama was less than 2.3%.
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