Causative agents of early male-killing phenomenon newly found in Homona magnanima
| Project/Area Number |
24580076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
KUNIMI Yasuhisa 東京農工大学, (連合)農学研究科(研究院), 教授 (50195476)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAI Madoka 東京農工大学, 大学院農学研究院, 教授 (60302907)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | オス殺し / Spiroplasma / チャハマキ / early male-killing / RNAウイルス / 有病率 / 混合感染 / Wolbachia / male-killing / 性比異常 / ウイルス |
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| Outline of Final Research Achievements |
The early male-killing strain of H. magnanima (Shizuoka strain) was collected in a tea field in Shizuoka, 2009. Purpose of this study was to identify the early male-killing agent. By running PCR with Wolbachia and Spiroplasma primers, the Shizuoka strain was infected with both bacteria. The strain which infected only Spiroplasma was produced by injecting larval hemolymph obtained from the Shizuoka strain to the Bacteria free strain which was established by continuously administering an antibiotic to the Shizuoka strain. No male was obtained from the Spiroplasma-infected strain, suggesting that the causative agent of early male-killing is Spiroplasma.
The generation changes in the prevalence of RNA virus (the late male-killing agent) and Spiroplasma in a natural population of H. magnanima were changed from 4.3 to 12.9% and 0.9 to 15.1%, respectively. Occurrence of H. magnanima infected with both RNA virus and Spiroplasama was less than 2.3%.
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Report
(4 results)
Research Products
(7 results)
