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Characterization of prostaglandin-producing enzyme aiming for insect sterilization

Research Project

Project/Area Number 24580078
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied entomology
Research InstitutionKyushu University

Principal Investigator

YAMAMOTO Koji  九州大学, (連合)農学研究科(研究院), 助教 (00346834)

Co-Investigator(Kenkyū-buntansha) SUZUKI Mamoru  大阪大学, たんぱく質研究所, 准教授 (40280507)
ARITAKE Kousuke  公益財団法人大阪バイオサイエンス研究所, 分子行動生物学部門, 研究員 (70390804)
HIGASHIURA Akifumi  大阪大学, たんぱく質研究所, 助教 (90598129)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsプロスタグランジン / プロスタグランジン合成酵素 / 応用昆虫
Outline of Final Research Achievements

Prostaglandins (PGs) are synthesized from arachidonic acid and influence a variety of physiological and pathological processes in mammalians. Knowledge on role and synthesis of PG in insect are limited, compared to mammalians. In this study, we identified PG synthase of Bombyx mori (PGES) and found that PGES produces E-type of PG (PGE). Since we were interested in the active sites in PGES, we determined crystal structure of PGES. PGES exists as homodimer and each monomer contains 10 alpha-helices and 4 beta-strands. By using site-directed mutagenesis, we Identified the amino acid residues (Trp39 and Gln63) involved in catalytic function.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results)

  • [Journal Article] Identification of residues essential for catalytic activity of a Bombyx mori arginase2014

    • Author(s)
      Hossain MDT, Nagaoka S, Yamamoto K
    • Journal Title

      Journal of Insect Biotechnology & Sericology

      Volume: 83 Pages: 47-51

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural characterization of the catalytic site of a Nilaparvata lugens delta-class glutathione transferase2014

    • Author(s)
      Yamamoto K, Higashiura A, Hossain MDT, Yamada N, Shiotsuki T, Nakagawa A
    • Journal Title

      Archives of Biochemistry and Biophysics

      Volume: 566 Pages: 36-42

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glutathione-binding site of a Bombyx mori theta-class glutathione transferase2014

    • Author(s)
      Hossain MDT, Yamada N, Yamamoto K
    • Journal Title

      PloS One

      Volume: 9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 特集「昆虫由来酵素・タンパク質の機能解析とその応用」にあたって2014

    • Author(s)
      山本幸治
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 83 Pages: 3-3

    • NAID

      130004948828

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 昆虫グルタチオン転移酵素群の機能と応用2014

    • Author(s)
      山本幸治
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 83 Pages: 5-10

    • NAID

      130004948829

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Crystal structure of a Bombyx mori Sigma-class glutathione transferase exhibiting prostaglandin E synthase activity2013

    • Author(s)
      Yamamoto K, Higashiura A, Suzuki M, Aritake K, Urade Y, Uodome N, Nakagawa A
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1830 Pages: 3711-3718

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] New insights into the catalytic mechanism of Bombyx mori prostaglandin E synthase gained from structure-function analysis2013

    • Author(s)
      Yamamoto K, Suzuki M, Higashiura A, Aritake K, Urade Y, Uodome N, Hossain MT, Nakagawa A
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 440 Pages: 762-797

    • Related Report
      2013 Research-status Report
  • [Journal Article] Cloning and characterization of a microsomal epoxide hydrolase from Heliothis virescens2013

    • Author(s)
      Shizuo G Kamita, Kohji Yamamoto, Mary M Dadala, Khavong Pha, Christophe Morisseau, Aurelie Escaich, Bruce Hammock
    • Journal Title

      Insect Biochem. Mol. Biol.

      Volume: 43 Pages: 219-228

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase2012

    • Author(s)
      Yamamoto K, Usuda K, Kakuta Y, Kimura M, Higashiura A, Nakagawa A, Aso Y, Suzuki M
    • Journal Title

      Biochim Biophys Acta-General Subjects

      Volume: 1820 Pages: 1469-1474

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] トビイロウンカ由来Delta-classグルタチオン転移酵素のX線立体構造解析2014

    • Author(s)
      山本 幸治, 東浦彰史, 山田直隆, 中川敦史
    • Organizer
      日本農芸化学会西日本支部講演会
    • Place of Presentation
      佐賀
    • Year and Date
      2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structural basis of catalytic mechanism of Bombyx mori prostaglandin E synthase2014

    • Author(s)
      Yamamoto K
    • Organizer
      9th International Workshop on the Molecular Biology and Genetics of the Lepidoptera
    • Place of Presentation
      Crete
    • Year and Date
      2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Crystal structure of a Bombyx mori Omega-class glutathione transferase2013

    • Author(s)
      Yamamoto K., Suzuki M., Higashiura A., Nakagawa A.
    • Organizer
      The 12th CONFERENCE of THE ASIAN CRYSTALLOGRAPHIC ASSOCIATION
    • Place of Presentation
      Hong Kong
    • Related Report
      2013 Research-status Report
  • [Presentation] プロスタグランジンE合成酵素の立体構造と機能2013

    • Author(s)
      山本 幸治
    • Organizer
      第37回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      長崎
    • Related Report
      2013 Research-status Report
  • [Presentation] Novel Glutathione Transferase in Lepidoptera

    • Author(s)
      Kohji Yamamoto
    • Organizer
      4th international symposium on pesticide and environmental safety
    • Place of Presentation
      Beijin (China)
    • Related Report
      2012 Research-status Report
  • [Presentation] デルタクラス・グルタチオン転移酵素の機能と構造

    • Author(s)
      山本幸治, 臼田一弘, 角田佳充, 木村誠, 東浦彰史, 中川敦史, 鈴木守
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] Crystal structure and characterization of glutathione transferase of the silkmoth

    • Author(s)
      Kohji Yamamoto
    • Organizer
      7th Wild Silkmoth and Silk
    • Place of Presentation
      Khon Kaen (Thailand)
    • Related Report
      2012 Research-status Report
  • [Presentation] 山本幸治, 伴野豊, 麻生陽一

    • Author(s)
      カイコ・ステロイド還元酵素のX線立体構造解析
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      つくば
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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