Proteomic analysis of the metabolic mechanism of entomopathogenic fungus for the application of bioinsecticide.
| Project/Area Number |
24580082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 昆虫病原性糸状菌 / スフィンゴシン / プロテオーム / 糸状菌 |
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| Outline of Final Research Achievements |
The mechanism of germination-acceleration of entomopathogenic fungus Nomuraea rileyi in the presence of C14-sphingosine was analyzed by proteomic technique. Twenty-one specific induced proteins were found by 2D electrophoresis on sphingosine induced germinated spore. By the analysis of these proteins by mass spectrometry, 15 proteins were identified and found their functions, and the other unknown 6 proteins were analyzed by further homology search to deduce their functions. The result showed that the specific developed proteins carried the function of metabolic enzymes and self-defense. The specific receptor for C14-sphingosine was now analyzing.
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Report
(4 results)
Research Products
(6 results)
