Construction of a base for utilization of insect cell-free protein synthesis system
| Project/Area Number |
24580083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Okinawa National College of Technology |
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Principal Investigator |
ITO Masaaki 沖縄工業高等専門学校, 生物資源工学科, 教授 (00395659)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 無細胞タンパク質合成系 / 昆虫 / カイコ / 翻訳促進配列 / チロシナーゼ / ラッカーゼ / 昆虫培養細胞 / 後部絹糸線 / 無細胞タンパク質合成 / 無細胞翻訳系 / バキュロウイルス |
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| Outline of Final Research Achievements |
In the present study, we analyzed an enhancer sequence composed of Spodoptera frugiperda nucleopolyhedrovirus polyhedorin 5’-UTR for improvement of Bombyx mori cell-free protein synthesis system. As a result, we found important sequence of 18 base length composed 3'-terminal region. A protein synthesis activity using the sequence as enhancer was 1.3-fold greater than that of using usual enhancer.
We constructed cell-free translation and analysis system with co-expression of activation protein using organic solvent resistant tyrosinase as a model protein. Furthermore, we also constructed membrane-binding protein expression and analysis system using thermostable laccase as a model protein.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] A cell-free translocation system using extracts of cultured insect cells to yield functional membrane proteins2014
Author(s)
Toru Ezure, Kei Nanatani, Satomi Suzuki, Yoko Sato, Keishi Aizawa, Satoshi Souma, Masaaki Ito, Takahiro Hohsaka, Gunnar von Heijine, Toshihiko Utsumi, Keietsu Abe, Eiji Ando, Nobuyuki Uozumi
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Journal Title
PLoS ONE
Volume: 9
Pages: 1-17
Related Report
Peer Reviewed / Open Access
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