| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In the present study, we analyzed an enhancer sequence composed of Spodoptera frugiperda nucleopolyhedrovirus polyhedorin 5’-UTR for improvement of Bombyx mori cell-free protein synthesis system. As a result, we found important sequence of 18 base length composed 3'-terminal region. A protein synthesis activity using the sequence as enhancer was 1.3-fold greater than that of using usual enhancer.
We constructed cell-free translation and analysis system with co-expression of activation protein using organic solvent resistant tyrosinase as a model protein. Furthermore, we also constructed membrane-binding protein expression and analysis system using thermostable laccase as a model protein.
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