| Project/Area Number |
24580085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
MIYAZAWA mitsuhiro 独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, ユニット長 (90370684)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Toshimasa 独立行政法人農業生物資源研究所, 先端ゲノム研究センター, 生体分子研究ユニット長 (40360458)
ISHIDA Yoko 神戸大学, 理学(系)研究科(研究院), グローバルCOE研究員 (90509861)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | フェロモン分解 / アルコールオキシダーゼ / アルコールデハイドロゲナーゼ / フェロモン分解酵素 / カイコ我 / カイコ蛾 / タンパク質 / 化学交信 / フェロモン / 分解酵素 |
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| Outline of Final Research Achievements |
Pheromone binding proteins (PBPs) and pheromone degradation enzymes (PDEs) play a part in chemical communication of insects. Although the PBP is unfolds widely, molecular mechanism analysis of PDE is still insufficient. In this study, we identified two estimated enzymes from the antenna of Japanese silkworm when it affected pheromone degradation. We purified these enzymes from approximately 3,000 antennae of the silkworm. However, the purest fraction separated by high resolution ion exchange chromatography contains several proteins in SDS-PAGE analysis. PDE from silkworm might be composed of various subunit.
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