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Elucidation of molecular mechanisms underlying avoidance of excess ammonium uptake into roots in rice

Research Project

Project/Area Number 24580090
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

HAYAKAWA Toshihiko  東北大学, (連合)農学研究科(研究院), 准教授 (60261492)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords遺伝子 / シグナル伝達 / 植物 / 生理学 / 発現制御 / 植物代謝調節
Outline of Final Research Achievements

Nitrogen is an essential macronutrient required in quantity for plant growth and productivity. Rice grown in flooded paddy fields chiefly takes up and uses an inorganic form of soil ammonium as an external nitrogen source; however, a sole supply of ammonium at high concentration frequently causes toxic effects to many terrestrial plants. Down-modulation of the high-affinity transport system for ammonium uptake into plant roots in response to an increasing ammonium supply is important for preventing ammonium-toxicity in plants. In this study, a protein kinase OsACTPK1 was suggested to be involved in negative modulation of ammonium transport activity of plasma membrane proteins associated with ammonium uptake in rice roots in response to an ascending external ammonium and possibly to internal carbon availability. This finding will provide a cue for better understanding of molecular mechanisms for preventing excess ammonium uptake into roots.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Invited: 2 results) Remarks (3 results)

  • [Journal Article] Asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots2015

    • Author(s)
      Ohashi M, Ishiyama K, Kojima S, Nakano K, Kanno K, Hayakawa T, Yamaya, T
    • Journal Title

      Plant and Cell Physiology

      Volume: 56 Issue: 4 Pages: 769-778

    • DOI

      10.1093/pcp/pcv005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Lack of cytosolic glutamine synthetase1;2 in vascular tissues of axillary buds caused severe reduction in their outgrowth and disorder of metabolic balance in rice seedlings2015

    • Author(s)
      Ohashi, M., Ishiyama, K., Kusano, M., Fukushima, A., Kojima, S., Hanada, A., Kanno, K., Hayakawa, T., Seto, Y., Kyozuka, J., Yamaguchi, S., Yamaya, T.
    • Journal Title

      Plant J.

      Volume: 81 Issue: 2 Pages: 347-356

    • DOI

      10.1111/tpj.12731

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root2014

    • Author(s)
      Soichi Kojima, Noriyuki Konishi, Marcel Pascal Beier, Keiki Ishiyama, Ikumi Maru, Toshihiko Hayakawa, Tomoyuki Yamaya
    • Journal Title

      Plant Signaling & Behavior

      Volume: 9 Issue: 8 Pages: 1-2

    • DOI

      10.4161/psb.29402

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NADH-dependent glutamate synthase plays a crucial role in assimilating ammonium in Arabidopsis root2014

    • Author(s)
      Konishi, N., Ishiyama, K., Matsuoka, K., Maru, I., Hayakawa, T., Yamaya, T. and Kojima, S.
    • Journal Title

      Physiologia Plantarum

      Volume: in press Issue: 1 Pages: 138-151

    • DOI

      10.1111/ppl.12177

    • NAID

      120005691730

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cytosolic glutamine synthetase1;2 is responsible for the primary assimilation of ammonium in rice roots2013

    • Author(s)
      Funayama, K.$, Kojima, S.$, Tabuchi-Kobayashi, M., Sawa, Y., Nakayama, Y., Hayakawa, T. and Yamaya, T.
    • Journal Title

      Plant and Cell Physiology

      Volume: 54 Issue: 6 Pages: 934-943

    • DOI

      10.1093/pcp/pct046

    • NAID

      10031184135

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Stimulation of axillary buds elongation by metabolite and cytokinin in rice2015

    • Author(s)
      Miwa Ohashi, Keiki Ishiyama, Miyako Kusano, Atsushi Fukushima, Mikiko Kojima, Soichi Kojima, Toshihiko Hayakawa, Hitoshi Sakakibara, Tomoyuki Yamaya
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of a specific combination of DNA elements in the promoter regions of ammonium responsive genes in Arabidopsis root2015

    • Author(s)
      Noriyuki Konishi, Keiki Ishiyama, Aoi Maruyama, Masahide Saito, Ikumi Maru, Toshihiko Hayakawa, Tomoyuki Yamaya, Soichi Kojima
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Down-regulation of ammonium uptake into plant roots under high concentration of external ammonium, 「Mineral transport and sensing in plants」2014

    • Author(s)
      Toshihiko Hayakawa, Tomoyuki Yamaya
    • Organizer
      日本土壌肥料学会2014年度東京大会シンポジウム
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] イネの根におけるAS分子種の生理的な役割と制御機構の解明2014

    • Author(s)
      大橋美和、石山敬貴、小島創一、早川俊彦、山谷知行
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高親和型尿素輸送担体が破壊されたイネの収量の解析2014

    • Author(s)
      藤田貴之、石山敬貴、早川俊彦、山谷知行、小島創一
    • Organizer
      第32回日本植物細胞分子生物学会大会
    • Place of Presentation
      アイーナ(いわて県民情報交流センター)、盛岡
    • Year and Date
      2014-08-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rice novel protein kinase involved in regulation of ammonium uptake into roots under high concentration of external ammonium2013

    • Author(s)
      Toshihiko Hayakawa, Mitsuhiro Obara, Akiko Taniai, Yuki Sawa, Jin Ishizawa, Soichi Kojima, Tomoyuki Yamaya
    • Organizer
      2nd International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Puerto Varas, Chile
    • Related Report
      2013 Research-status Report
  • [Presentation] 植物の三大栄養素(N-P-K)の感知と利用の新理解、イネ根におけるアンモニウム態窒素吸収の負の制御の新理解

    • Author(s)
      早川俊彦、山谷知行
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] シロイヌナズナは2つの低親和型グルタミン合成酵素を使い分けて根でアンモニウムを同化する

    • Author(s)
      小西範幸、石山敬貴、丸郁美、松岡香矢、早川俊彦、山谷知行、小島創一
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Strigolactone-independent reduction of tiller number in rice mutant lacking cytosolic glutamine synthetase1;2 or NADH-glutamate synthase1

    • Author(s)
      Miwa Ohashi, Keiki Ishiyama, Atsushi Hanada, Soichi Kojima, Toshihiko Hayakawa, Yoshiya Seto, ShinjiroYamaguchi, Tomoyuki Yamaya
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] シロイヌナズナNADH-グルタミン酸合成酵素の根におけるアンモニウム同化の重要性

    • Author(s)
      小西範幸
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] シロイヌナズナ環境型におけるアンモニウム栄養利用の遺伝的な差異

    • Author(s)
      松岡香矢
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] シロイヌナズナサイトゾル型グルタミン合成酵素1;2は根でアンモニウムによって誘導されアンモニウムを同化する

    • Author(s)
      小西範幸
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Related Report
      2012 Research-status Report
  • [Remarks] 東北大学大学院農学研究科応用生命科学専攻植物機能科学講座植物細胞生化学分野ホームページ

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東北大学大学院農学研究科応用生命科学専攻植物機能科学講座植物細胞生化学分野ホームページ

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2013 Research-status Report
  • [Remarks] 東北大学大学院農学研究科応用生命科学専攻植物機能科学講座植物細胞生化学分野ホームページ

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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