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Basic research for accumulating oil in plant vegetative tissues

Research Project

Project/Area Number 24580091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTokyo Institute of Technology

Principal Investigator

SHIMOJIMA Mie  東京工業大学, バイオ研究基盤支援総合センター, 助教 (90401562)

Co-Investigator(Renkei-kenkyūsha) 太田 啓之  東京工業大学, バイオ研究基盤支援総合センター, 教授 (20233140)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords植物脂質 / 貯蔵脂質 / トリアシルグリセロール / リン欠乏
Outline of Final Research Achievements

We produced Arabidopsis transgenic plants overexpressing one of the key enzymes in TAG synthesis (diacylglycerol acyltransferase 1, 2 and phospholipid: diacylglycerol acyltransferase 1) during phosphate starvation by utilizing a phosphate-deficiency responsive promoter (AtMGD3 promoter) using wild-type or pgm-1 as genetic backgrounds for transformation. The produced transgenic plants accumulated large amount of oil in shoots under phosphate-depleted conditions, whereas the effect was different from the results obtained from roots. From these results, it was suggested that the mechanism of oil accumulation during phosphate starvation was different between shoots and roots.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2014 2013 Other

All Presentation (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Presentation] Engineering the lipid remodeling system during phosphate starvation enhances oil accumulation in plant vegetative tissues2014

    • Author(s)
      下嶋美恵、円由香、太田啓之
    • Organizer
      21th International Symposium on Plant Lipids
    • Place of Presentation
      Guelph, Ontario, Canada
    • Year and Date
      2014-07-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] リン欠乏条件を活用した植物葉における油脂増産系の構築

    • Author(s)
      下嶋 美恵
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山県富山市、富山大学
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 融合遺伝子、ベクター、トランスジェニック植物、植物油脂の製造方法、トランスジェニック植物の作出方法、およびトランスジェニック植物の作出用キット2014

    • Inventor(s)
      太田啓之、下嶋美恵、円由香
    • Industrial Property Rights Holder
      東京工業大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-08-26
    • Related Report
      2014 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 融合遺伝子、ベクター、トランスジェニック植物、植物油脂の製造方法、トランスジェニック植物の作出方法、およびトランスジェニック植物の作出用キット2013

    • Inventor(s)
      太田啓之、下嶋美恵、円由香
    • Industrial Property Rights Holder
      東京工業大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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