Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
We improved a method for functional analysis of transporter proteins based on liposome-supplemented cell-free system. In the new system, detection range of transport activity against non-specific background activity was improved by up-regulated synthetic efficiency of membrane proteins in cell-free reaction. By using this method, we analyzed several transporter proteins localized in plant organelle. Substrate specificity of sugar-phosphate transporters in rice and iceplant plastids and sulfate metabolite transporter in Arabidopsis plastids.
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