Functional analysis of transporters regulating translocation of energy metabolites in rice organelle
| Project/Area Number |
24580094
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Ehime University |
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Principal Investigator |
NOZAWA Akira 愛媛大学, プロテオサイエンスセンター, 講師 (30432800)
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| Co-Investigator(Renkei-kenkyūsha) |
TOZAWA Yuzuru 埼玉大学, 大学院・理工学研究科, 教授 (90363267)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖リン酸輸送体 / ミトコンドリアキャリア / コムギ無細胞系 / リポソーム / トランスポーター / 無細胞タンパク質合成系 / オルガネラ |
|---|
| Outline of Final Research Achievements |
We improved a method for functional analysis of transporter proteins based on liposome-supplemented cell-free system. In the new system, detection range of transport activity against non-specific background activity was improved by up-regulated synthetic efficiency of membrane proteins in cell-free reaction. By using this method, we analyzed several transporter proteins localized in plant organelle. Substrate specificity of sugar-phosphate transporters in rice and iceplant plastids and sulfate metabolite transporter in Arabidopsis plastids.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant <i>Mesembryanthemum crystallinum</i>2013
Author(s)
Kore-eda, S., Nozawa, A., Okada, Y., Takashi, K., Azad, M.A.K., Ohnishi, J., Nishiyama, Y. and Tozawa, Y.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 7
Pages: 1511-1516
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Presentation] Functional analysis of membrane transporters by in vitro reconstitution system2014
Author(s)
Daichi Morita, Hiroyuki Inoue, Yuki Shimonosono, Hitoshi Myoraku, Taito Takabayashi, Ryoji Fujimoto, Nobuhiro Yamashita, Yusuke Okada, Shinichi Teramoto, Hironori Takeda, Akira Nozawa and Yuzuru Tozawa
Organizer
The 12th Matsuyama International Symposium on Proteo-Sciences
Place of Presentation
愛媛大学法文学部講義棟・愛媛県・松山市
Year and Date
2014-09-17
Related Report
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