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The mechanism of electron partitioning between carbon fixation and nitrogen assimilation via isoforms of FNR and ferredoxin

Research Project

Project/Area Number 24580098
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Higuchi Mieko  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (40443014)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords炭酸固定 / 窒素代謝 / 光合成 / 窒素固定 / 炭素代謝 / FNR / 光合成電子伝達 / 二酸化炭素固定
Outline of Final Research Achievements

In this study, I have investigated the gene expression profiles of Fd and FNR isoforms using Arabidopsis and rice plants. Gene expression profiles under elevated CO2 condition demonstrated that expressions of FNR1, FNR2, Fd1 and Fd2 were decreased and Fd3 and Fd4 expressions were increased. This suggests that stoichiometry of Fd isoforms might be involved in electron partition to CO2 fixation under elevated CO2 condition. Gene expression profile using rice FNR1 and FNR2 overexpression plants revealed that expression of genes related to nitrogen metabolism were decreased only in FNR2 overexpression plants. This result suggests that electron might be preferentially transferred to nitrogen assimilation pathway in FNR2 overexpression plants whereas linear photosynthetic electron transport is not impaired in FNR1 overexpression plants.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Substantial expression of novel small open reading frames in Oryza sativa2014

    • Author(s)
      Okamoto M, Higuchi-Takeuchi M, Shimizu M, Shinozaki K, Hanada K
    • Journal Title

      Plant Signal Behav.

      Volume: 9 Issue: 2 Pages: e27848-e27848

    • DOI

      10.4161/psb.27848

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] BPG3 is a novel chloroplast protein that involves the greening of leaves and related to brassinosteroid signaling2014

    • Author(s)
      Yoshizawa E, Kaizuka M, Yamagami A, Higuchi-Takeuchi M, Matsui M, Kakei Y, Shimad Y, Sakuta M, Osada H, AsamT, Nakano T
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 印刷中 Issue: 3 Pages: 420-429

    • DOI

      10.1080/09168451.2014.885831

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The RNA-binding protein FPR regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation2013

    • Author(s)
      Lyons R, Iwase A, Gaensewig T, Sherstnev A, Duc C, Barton G, Hanada K, Higuchi-Takeuchi M, Matsui M, Sugimoto K, Kazan K, Simpson G, and Shirasu K
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 2866-2866

    • DOI

      10.1038/srep02866

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Small open reading frames associated with morphogenesis are hidden in plant genomes2013

    • Author(s)
      Hanada K, Higuchi-Takeuchi M, Okamoto M, Yoshizumi T, Shimizu M, Nakaminami K, Nishi R, Ohashi C, Iida K, Tanaka M, Horii Y, Kawashima M, Matsui K, Toyoda T, Shinozaki K, Seki M, Matsui M.
    • Journal Title

      PNAS

      Volume: 110 Pages: 2395-2400

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Synthesis of polyhydroxyalkanoates by photosyntjetic purple bacteria.2015

    • Author(s)
      Higuchi-Takeuchi M, Keiji Numata
    • Organizer
      The international chemical congress of PACIFIC BASIN SOCIETIES
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of polyhydroxyalkanoates (PHAs) production in photosynthetic purple bacteria.2015

    • Author(s)
      Higuchi-Takeuchi M, Keiji Numata
    • Organizer
      7th international conference on Green and Sustainable Chemistry
    • Place of Presentation
      一ツ橋ホール・千代田区・東京
    • Year and Date
      2015-07-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ポリヒドロキシアルカン酸を生産する海洋性光合成細菌の同定2015

    • Author(s)
      樋口美栄子、沼田圭司
    • Organizer
      高分子学会
    • Place of Presentation
      札幌コンベンションセンター・札幌市・北海道
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 海洋性光合成細菌によるポリヒドロキシアルカン酸(PHA)の生産2015

    • Author(s)
      樋口美栄子、沼田圭司
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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