Role of genistein-induced multidrug efflux pump of Bradyrhizobium japonicum on symbiosis with soybean
Project/Area Number |
24580099
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
Principal Investigator |
OHWADA Takuji 帯広畜産大学, 畜産学部, 教授 (90211804)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | ダイズ根粒菌 / 多剤排出ポンプ / ゲニステイン / 相利共生 / bradyrhizobium / genistein / multidrug efflux pump / tetR family |
Outline of Final Research Achievements |
One genomic region, which is induced by genistein, was found in Bradyrhizobium japonicum and named BjG30. The induction level reached maximum at 15 min after the addition of genistein and the induction profile was different from that of symbiosis genes (nod genes). The BjG30 composed of multidrug efflux pump, polyhydroxybutyrate metabolism, and transcriptional regulatory genes, and played an important role in symbiosis with soybean for the nodule formation and nitrogen fixation. In addition, the results of global gene expression analyses showed that the efflux of genistein would functionally regulate the expression levels of symbiosis genes.
|
Report
(4 results)
Research Products
(5 results)
-
[Journal Article] Involvement of a Novel Genistein-Inducible Multidrug Efflux Pump of <i>Bradyrhizobium japonicum</i> Early in the Interaction with <i>Glycine max</i> (L.) Merr2013
Author(s)
K.Takeshima, T.Hidaka, M.Wei, T.Yokoyama, K.Minamisawa, H.Mitsui, M.Itakura, T.Kaneko, S.Tabata, K.Saeki, H.Oomori, S.Tajima, T.Uchiumi, M.Abe, Y.Tokuji, and T.Ohwada
-
Journal Title
Microbes and Environments
Volume: 28
Issue: 4
Pages: 414-421
DOI
NAID
ISSN
1342-6311, 1347-4405
Related Report
Peer Reviewed
-
-
-
-