Elucidation of a whole transcriptional control network concerning environmental response by creation of an artificial ancestor cell
| Project/Area Number |
24580101
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Saitama University |
|---|
Principal Investigator |
ASAI Kei 埼玉大学, 理工学研究科, 准教授 (70283934)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YOSHIKAWA Hirofumi 東京農業大学, 応用生物科学部, 教授 (50175676)
|
|---|
| Project Period (FY) |
2012-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 転写制御 / シグマ因子 / 細胞死 / ストレス応答 / 枯草菌 |
|---|
| Outline of Final Research Achievements |
We aimed at elucidation of whole transcriptional control mechanism for bacterial stress response. The bacillus cells where 18 sigma factors out of all 19 were inactivated was created (the stain of sigma-A only) and it was revealed that only one essential sigma factor was enough to grow. The vegetative growth of the stain of sigma-A only is identical to that of the wild type strain in a nutritionally rich medium, but interestingly the turbidity of the cells are reduced after the exponential growth phase suggesting that autolysis of the cells is occurred. Suppressor mutants which escaped cell lysis were obtained and their location of mutation was analyzed. Together all this data, it was suggested that novel mechanism for cell survival at stationary phase, in which transcription and translation were closely related.
|
Report
(5 results)
Research Products
(27 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 枯草菌αオペロンの構造と発現の解析2015
Author(s)
白川文教、池田宗太郎、朝井 計
Organizer
第9回日本ゲノム微生物学会年会
Place of Presentation
神戸大学六甲第2キャンパス(神戸市灘区六甲台町)
Year and Date
2015-03-06 – 2015-03-08
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
