The prokaryotic Hsp90 chaperone network
Project/Area Number |
24580102
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Saitama University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 分子シャペロン / 熱ショックタンパク質 / Hsp90 / Hsp70 / シアノバクテリア / HtpG / DnaK / DnaJ / GroEL / シャペロンネットワーク / Hsp40 / 協同的シャペロン作用 |
Outline of Final Research Achievements |
HtpG, a bacterial heat shock protein 90 (Hsp90), is essential for thermotolerance in some prokaryotes. HtpG functioned with DnaK2 (Hsp70)/DnaJ2/GrpE to assist unfolding/folding of denatured proteins in both ATP-dependent and -independent fashions. The cooperative action of HtpG and DnaK2 might play a key role under stress. DnaJ2 and GroEL also interacted with HtpG physically. We found that a naturally occurring styryl-lactone goniothalamin activates the ATPase activity of Hsp90. Unexpectedly, it inhibited the activity of HtpG that assists refolding of a non-native protein in cooperation with the DnaK2 chaperone system. It indicates that the ATPase activity of HtpG has to be controlled so that it can co-operate with DnaK2 effectively.
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Report
(5 results)
Research Products
(39 results)
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[Journal Article] Physical Interaction between Bacterial Heat Shock Protein 90 (Hsp90) and Hsp70 Chaperones Mediates Their Cooperative Action to Refold Denatured Proteins2014
Author(s)
Hitoshi Nakamoto, Kensaku Fujita, Aguru Ohtaki, Satoru Watanabe, Shoichi Narumi, Takahiro Maruyama, Emi Suenaga, Tomoko S. Misono, Penmetcha K.R. Kumar, Pierre Goloubinoff, Hirofumi Yoshikawa
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Journal Title
The Journal of Biological Chemistry
Volume: 289
Issue: 9
Pages: 6110-6119
DOI
Related Report
Peer Reviewed
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[Presentation] シアノバクテリアHsp70 (DnaK2)とHsp90 (HtpG)及びリン脂質との特異的相互作用2014
Author(s)
Hitoshi Nakamoto, Kensaku Fujita, Aguru Ohtaki, Saaimatul Huq, Keigo Sueoka, Viktoria Varvasovszki, Attila Glatz, Zsolt Torok, Ana-Maria Pilbat, Ibolya Horvath, Laszlo Vigh
Organizer
第37回日本分子生物学会年会
Place of Presentation
パシフィコ横浜
Year and Date
2014-11-25 – 2014-11-27
Related Report
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[Presentation] Amphitropic DnaK2: Its association with Hsp90 and lipid membrane in cyanobacteria2014
Author(s)
Hitoshi Nakamoto, Kensaku Fujita, Aguru Ohtaki, Saaimatul Huq, Keigo Sueoka, Viktoria Varvasovszki, Attila Glatz, Zsolt Torok, Ana-Maria Pilbat, Ibolya Horvath, Laszlo Vigh
Organizer
FASEB Science Research Conference ‘Protein Folding in the Cell’
Place of Presentation
Vermont, 米国
Year and Date
2014-07-20 – 2014-07-25
Related Report
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