Elucidation of the role of aromatic amino acid residues in crystalline chitin hydrolysis and development to the mechanism of a-chitin hydrolysis
| Project/Area Number |
24580104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IGARASHI Kiyohiko 東京大学, 大学院農学生命科学研究科, 准教授 (80345181)
SUZUKI Kazushi 新潟大学, 自然科学系, 准教授 (00444183)
SUGIMOTO Hayuki 新潟大学, 自然科学系, 助教 (60529527)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | キチナーゼ / 結晶性キチン分解 / Serratia marcescens / 高速原子間力顕微鏡 / 芳香族アミノ酸残基 / 結晶性キチン |
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| Outline of Final Research Achievements |
Chitin is a crystalline and rigid structural polysaccharide present in a variety of organisms. Elucidation of the mechanism underlying in the enzymatic degradation of crystalline chitin is very important not only from enzymological and biological view points, but also for utilization of biomass resources. In this study, critical roles of the aromatic amino acid residues, which are present on the surface of chitinase molecule and inside of its catalytic cleft, in processive hydrolysis of crystalline chitin was demonstrated.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin2014
Author(s)
Igarashi, K., Uchihashi, T., Uchiyama, T., Sugimoto, H., Wada, M., Suzuki, K., Sakuda, S., Ando, T., Watanabe, T., and Samejima, M.
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Journal Title
Nature Commun.
Volume: 5
Issue: 1
Pages: 3975-3975
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Studies on mechanism of crystalline chitin hydrolysis by Serratia marcescens chitinase B using high-speed atomic force microscopy.2013
Author(s)
Sugimoto, K. Nakamura, Y. Nishino, A. Fujinuma, H. Watanabe, T. Uchihashi, T. Ando, K. Igarashi, M. Wada, M. Samejima, K. Suzuki, and T. Watanabe
Organizer
10th Asia-Pacific Chitin and Chitosan Symposium / 27th Japanese Chitin and Chitosan Symposium.
Place of Presentation
米子コンベンションセンター
Related Report
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