| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Streptococcus mutans, a tooth decay bacterium, has an unusual glycolytic pathway that can generate NADPH at the step of glyceraldehyde 3-phosphate dehydrogenase. We engineered Corynebacterium glutamicum with an S. mutans-type NADPH-generating glycolytic pathway. A lysine producer derived from the engineered strain produced more than twice as much lysine as the reference strain. The production of lysine remained high after the pentose phosphate pathway was interrupted, while a drastic decrease in lysine production was observed in the case of the reference strain.
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