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Development of a Corynebacterium glutamicum strain with an NADPH-generating glycolytic pathway

Research Project

Project/Area Number 24580105
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionShinshu University

Principal Investigator

IKEDA Masato  信州大学, 学術研究院農学系, 教授 (00377649)

Co-Investigator(Kenkyū-buntansha) TAKENO Seiki  信州大学, 学術研究院農学系, 助教 (30422702)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsコリネバクテリウム・グルタミクム / 還元力 / NADPH / GapN / リジン発酵 / rho
Outline of Final Research Achievements

Streptococcus mutans, a tooth decay bacterium, has an unusual glycolytic pathway that can generate NADPH at the step of glyceraldehyde 3-phosphate dehydrogenase. We engineered Corynebacterium glutamicum with an S. mutans-type NADPH-generating glycolytic pathway. A lysine producer derived from the engineered strain produced more than twice as much lysine as the reference strain. The production of lysine remained high after the pentose phosphate pathway was interrupted, while a drastic decrease in lysine production was observed in the case of the reference strain.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013 2012 Other

All Presentation (5 results) (of which Invited: 3 results) Book (3 results)

  • [Presentation] ミュータンス菌型のレドックス代謝をもつ還元力高産生コリネ菌の開発2015

    • Author(s)
      竹野誠記、見村晃紀、三橋 敏、池田正人
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 還元力高産生コリネ菌によるペントース-リン酸経路非依存リジン発酵2015

    • Author(s)
      堀 一将、大谷幸子、三橋 敏、竹野誠記、池田正人
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] アミノ酸を生み出す菌の作り方

    • Author(s)
      池田正人
    • Organizer
      第3回有用微生物応用研究会
    • Place of Presentation
      長野市ものづくり支援センター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Design of Streptococcus mutans-type redox metabolism in Corynebacterium glutamicum.

    • Author(s)
      Ikeda M
    • Organizer
      The 13th Swiss-Japanese Joint Meeting on Biotechnology and Bioprocess Development
    • Place of Presentation
      Walzenhausen, Switzerland
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Engineering of Glutamic Acid Bacteria on the model of the unique redox metabolism of Tooth Decay Bacteria.

    • Author(s)
      Ikeda M
    • Organizer
      15th International Biotechnology Symposium and Exhibition (IBS2012)
    • Place of Presentation
      Daegu, Korea
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 生命のビッグデータ利用の最前線2014

    • Author(s)
      池田正人 他
    • Total Pages
      231
    • Publisher
      シーエムシー出版
    • Related Report
      2014 Annual Research Report
  • [Book] Microbiology monographs 23, Corynebacterium glutamicum2013

    • Author(s)
      Ikeda M, Takeno S
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report
  • [Book] 微生物を活用した新世代の有用物質生産技術2012

    • Author(s)
      池田正人
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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