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Production of useful saccharides from chitin using novel chitinolytic enzymes

Research Project

Project/Area Number 24580107
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionShinshu University

Principal Investigator

SHIMOSAKA Makoto  信州大学, 学術研究院繊維学系, 教授 (90187477)

Co-Investigator(Kenkyū-buntansha) TAGUCHI Goro  信州大学, 学術研究院繊維学系, 准教授 (70252070)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsキチナーゼ / キチン / N-アセチルグルコサミン / 糖質加水分解酵素 / 遺伝子破壊 / キチン分解細菌 / Nーアセチルグルコサミン / Nーアセチルグルコサミン / キチン結合ドメイン
Outline of Final Research Achievements

The novel chitinolytic bacterium, Chitiniphilus shinanonensis strain SAY3 was isolated and identified. Draft sequence of SAY3 genomic DNA revealed a presence of 49 putative genes coding for enzymes related to chitin degradation. Investigation of the recombinant proteins obtained by expression of these genes in Escherichia coli clarified novel types of chitinolytic enzymes as follows. The enzyme ChiG catalyzed an endo-type cleavage of chitin, however, it gave N-acetylglucosamine (GlcNAc) alone as a final product. ChiJ and ChiK catalyzed a processive-type reaction releasing GlcNAc dimer from the reducing-end of substrates. ChiL predominantly exhibited a trans-glycosylation activity. Chi33 is a lytic polysaccharide monooxygenase rather than a glycosyl hydrolase. Disruption of objective genes mediated by homologous recombination was achieved, which would be a promising tool to clarify a physiological function of the proteins encoded by unknown genes.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (8 results) (of which Invited: 1 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] キチン分解細菌Chitiniphilus shinanonensis由来の新規キチン分解酵素を用いたN-アセチルグルコサミンの生産2013

    • Author(s)
      下坂 誠
    • Journal Title

      バイオインダストリー

      Volume: 30 Pages: 52-59

    • Related Report
      2012 Research-status Report
  • [Journal Article] Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium <I>Chitiniphilus shinanonensis</I>2012

    • Author(s)
      Lanxiang Huang, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 3 Pages: 517-522

    • DOI

      10.1271/bbb.110822

    • NAID

      10030750647

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] キチン分解細菌Chitiniphilus shinanonensisにおける遺伝子操作系の開発2014

    • Author(s)
      園田 紀恵、兪 佳怡、野川 優洋、田口 悟郎、下坂 誠
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス (神奈川県川崎市)
    • Related Report
      2013 Research-status Report
  • [Presentation] キチン分解細菌Chitiniphilus shinanonensisが保有するキチン分解酵素ChiJ,ChiKの解析2014

    • Author(s)
      中野 萌, 兪 佳怡, 野川 優洋, 田口 悟郎, 下坂 誠
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス (神奈川県川崎市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Isolation and characterization of genes encoding chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis2013

    • Author(s)
      Makoto Shimosaka, Lanxiang Huang, Norie Sonoda, Moe Nakano, Masahiro Nogawa, Goro Taguchi
    • Organizer
      10th International Conference of the Asian Pacific Chitin Chitosan Symposium
    • Place of Presentation
      米子コンベンションセンター (鳥取県米子市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis -Development of gene disruption by an allelic exchange system-2013

    • Author(s)
      Norie Sonoda, Jiayi Yu, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka
    • Organizer
      10th International Conference of the Asian Pacific Chitin Chitosan Symposium
    • Place of Presentation
      米子コンベンションセンター (鳥取県米子市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis -chiG, chiJ, and chiK coding for unique chitin-degrading enzymes-2013

    • Author(s)
      Moe Nakano, Jiayi Yu, Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka
    • Organizer
      10th International Conference of the Asian Pacific Chitin Chitosan Symposium
    • Place of Presentation
      米子コンベンションセンター (鳥取県米子市)
    • Related Report
      2013 Research-status Report
  • [Presentation] キチン分解細菌Chitiniphilus shinanonensisに由来する新規キチナーゼについて2012

    • Author(s)
      黄 蘭香、鎮目有玲紗、野川優洋、田口悟朗、下坂 誠
    • Organizer
      第26回キチン・キトサンシンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] キチン分解細菌Chitiniphilus shinanonensisに由来するキチナーゼChiLが示す糖転移活性の解析2012

    • Author(s)
      園田 紀恵、中野 萌、鎮目 有玲紗、野川 優洋、田口 悟朗、下坂 誠
    • Organizer
      第64回日本生物工学会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] キチン分解細菌Chitiniphilus shinanonensisに由来する新規キチナーゼChiGの解析2012

    • Author(s)
      中野 萌、園田紀恵、鎮目有玲紗、黄 蘭香、野川優洋、田口悟朗、下坂 誠
    • Organizer
      第64回日本生物工学会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Remarks] 下坂研究室ホームページ

    • URL

      http://fiber.shinshu-u.ac.jp/biotech/home.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 下坂研究室ホームページ

    • URL

      http://fiber.shinshu-u.ac.jp/biotech/home

    • Related Report
      2013 Research-status Report
  • [Remarks] 下坂研究室ホームページ

    • URL

      http://www.fiber.shinshu-u.ac.jp/biotech/home.html

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] キチン分解酵素遺伝子および該遺伝子によりコードされるキチン分解酵素2012

    • Inventor(s)
      下坂 誠、田口悟朗、野川優洋
    • Industrial Property Rights Holder
      信州大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-08-23
    • Related Report
      2012 Research-status Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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