Functional analysis and application of Sun family proteins in filamentous fungi
| Project/Area Number |
24580108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 糸状菌 / 細胞壁 / 恒常性 / 分泌酵素生産 / ＳＵＮタンパク質 / SUN familyタンパク質 / 分泌酵素 / Sun family |
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| Outline of Final Research Achievements |
To clarify the physiological function of the sunA gene in Aspergillus nidulans, phenotypic characteristics of the sunA deletant and overexpressor were analyzed. The deletant displayed morphological abnormality of the mycelia, decreased conidiation, increased sensitivity to Calucofluor White and Cong Red. Furthermore, increased production of the secreted enzymes and leakage of intracellular proteins were evident. Expression profile of the genes related to beta-1,3-glucan synthesis and modification was also different from that of the wild type. The overexpressor did not show significant changes in the above phenotypes. The resulsts suggest that SunA is involved in bete-1,3-glucan homeostasis of the cell wall.
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Report
(4 results)
Research Products
(1 results)
