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Molecular characterization of acrylic acid production related genes and its application for bioprocess of acrylic acid production

Research Project

Project/Area Number 24580110
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionKyoto Institute of Technology

Principal Investigator

ASO Yuji  京都工芸繊維大学, 工芸科学研究科, 准教授 (70380590)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsアクリル酸 / 代謝工学 / 発酵工学 / バイオベースマテリアル
Outline of Final Research Achievements

The pct and lcdCAB genes from Megasphaera elsdenii JCM1772 were expressed in Escherichia coli BW25113 (DE3, ΔpflA). The transformant was cultivated in M9 medium, resulting in 0.17 mM acrylic acid production after 18 h cultivation. A similar experiment was done using Lactococcus lactis NZ9000 (L-ldh::D-ldh). However, no production of acrylic acid was confirmed in the engineered strain. As a reason for that, the expression levels of Pct and LcdCAB were low.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (28 results)

All 2015 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results) Presentation (17 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Photosynthetic production of itaconic acid in Synechocystis sp. PCC68032015

    • Author(s)
      T. Chin, M. Sano, T. Takahashi, H. Ohara, Y. Aso
    • Journal Title

      Journal of Biotechnology

      Volume: 195 Pages: 43-45

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A simple TLC–densitometric method for the quantification of acrylic acid in aqueous solutions2015

    • Author(s)
      M. Sano, T. Chin, T. Takahashi, H. Ohara, Y. Aso
    • Journal Title

      Journal of Planar Chromatography

      Volume: 28 Pages: 12-16

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of itaconic acid in Escherichia coli expressing recombinant α-amylase using starch as substrate2015

    • Author(s)
      S. Okamoto, T. Chin, K. Nagata, T. Takahashi, H. Ohara, Y. Aso
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 119 Pages: 548-553

    • NAID

      110009962609

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 微生物によるビニルモノマー生産2015

    • Author(s)
      麻生祐司
    • Journal Title

      化学工学

      Volume: 79 Pages: 307-309

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Production of itaconic acid using metabolically engineered Escherichia coli2014

    • Author(s)
      S. Okamoto, T. Chin, K. Hiratsuka, Y. Aso, Y. Tanaka, T. Takahashi, H. Ohara
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 60 Pages: 191-197

    • NAID

      130004713117

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 細菌のアクリル酸代謝2014

    • Author(s)
      麻生祐司
    • Journal Title

      生物工学会誌

      Volume: 92 Pages: 115-115

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 機能性乳酸菌の探索と高付加価値食品への応用2014

    • Author(s)
      麻生祐司
    • Journal Title

      食品加工技術

      Volume: 34 Pages: 171-177

    • NAID

      40020407255

    • Related Report
      2014 Annual Research Report
  • [Journal Article] バイオプロセスによるビニルモノマーの生産2013

    • Author(s)
      麻生祐司、陳泰駿、小原仁実
    • Journal Title

      ケミカルエンジニヤリング

      Volume: 58(8) Pages: 9-15

    • Related Report
      2013 Research-status Report
  • [Journal Article] 微生物発酵によるビニルモノマー生産法の開発2013

    • Author(s)
      麻生祐司、岡本脩佑、田中康智、小原仁実
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 71 Pages: 40-42

    • NAID

      10031140302

    • Related Report
      2012 Research-status Report
  • [Journal Article] バイオマスからのビニルモノマー生産とバイオベースポリマーへの展開2012

    • Author(s)
      麻生祐司、石本聖明、小原仁実、小林四郎
    • Journal Title

      バイオプラジャーナル

      Volume: 47 Pages: 16-23

    • Related Report
      2012 Research-status Report
  • [Presentation] 代謝改変大腸菌を用いたイコタン酸高発酵生産法の開発2015

    • Author(s)
      永田圭介、麻生祐司、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山市
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸菌を用いたアクリル酸発酵生産法の開発2015

    • Author(s)
      橋本彩加、麻生祐司、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山市
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] TLCを用いた環境水中アクリル酸の簡便定量法の開発2015

    • Author(s)
      佐野芽生、陳泰駿、麻生祐司、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山市
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 代謝工学に基づいた大腸菌によるイタコン酸生産2014

    • Author(s)
      麻生祐司、陳泰駿、平塚賢、小原仁実
    • Organizer
      日本生物工学会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] シアノバクテリアを用いた二酸化炭素からのイタコン酸生産2014

    • Author(s)
      陳泰駿、佐野芽生、麻生祐司、小原仁実
    • Organizer
      日本生物工学会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 固定化大腸菌を用いたイタコン酸生産システムの開発2014

    • Author(s)
      平塚賢、陳泰駿、麻生祐司、小原仁実
    • Organizer
      日本生物工学会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物プロセスによるバイオマスからのビニルモノマー生産

    • Author(s)
      麻生祐司、岡本脩佑、田中康智、陳泰駿、平塚賢、小原仁実
    • Organizer
      高分子学会
    • Place of Presentation
      金沢
    • Related Report
      2013 Research-status Report
  • [Presentation] Production of vinyl monomers from biomass by bioprocess

    • Author(s)
      Yuji Aso, Taejun Chin, Hitomi Ohara
    • Organizer
      Eco-Energy and Materials Science and Engineering Symposium
    • Place of Presentation
      プーケット、タイ
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 代謝工学に基づいた大腸菌によるイタコン酸生産

    • Author(s)
      麻生祐司、岡本脩佑、陳泰駿、平塚賢、田中康智、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] シアノバクテリアを用いた二酸化炭素からのイタコン酸生産

    • Author(s)
      陳泰駿、岡本脩佑、麻生祐司、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] バイオプロセスによるビニル化合物の生産

    • Author(s)
      麻生祐司
    • Organizer
      日本繊維機械学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] バイオプロセスによるビニルモノマー生産

    • Author(s)
      麻生祐司
    • Organizer
      高分子学会 エコマテリアル研究会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 微生物プロセスによるバイオマスからのビニルモノマー生産

    • Author(s)
      麻生祐司、岡本脩佑、田中康智、小原仁実
    • Organizer
      第61回高分子討論会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] Production of biobased chemicals by bacterial process

    • Author(s)
      Yuji Aso
    • Organizer
      10th Eco-Energy and Materials Science and Engineering Symposium
    • Place of Presentation
      タイ
    • Related Report
      2012 Research-status Report
  • [Presentation] 代謝工学に基づいた大腸菌によるイタコン酸生産

    • Author(s)
      岡本脩佑、田中康智、麻生祐司、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] アクリル酸生産関連遺伝子の発現とアクリル酸発酵生産への応用

    • Author(s)
      田中康智、岡本脩佑、麻生祐司、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] 大腸菌におけるBacillus amyloliquefaciens由来アミラーゼ遺伝子amyA発現系の構築

    • Author(s)
      麻生祐司、岡本脩佑、田中康智、小原仁実
    • Organizer
      日本農芸化学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Remarks] 生物資源システム工学研究室ホームページ

    • URL

      http://bio.eng.kit.ac.jp/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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