Molecular characterization of acrylic acid production related genes and its application for bioprocess of acrylic acid production
| Project/Area Number |
24580110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
ASO Yuji 京都工芸繊維大学, 工芸科学研究科, 准教授 (70380590)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | アクリル酸 / 代謝工学 / 発酵工学 / バイオベースマテリアル |
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| Outline of Final Research Achievements |
The pct and lcdCAB genes from Megasphaera elsdenii JCM1772 were expressed in Escherichia coli BW25113 (DE3, ΔpflA). The transformant was cultivated in M9 medium, resulting in 0.17 mM acrylic acid production after 18 h cultivation. A similar experiment was done using Lactococcus lactis NZ9000 (L-ldh::D-ldh). However, no production of acrylic acid was confirmed in the engineered strain. As a reason for that, the expression levels of Pct and LcdCAB were low.
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Report
(4 results)
Research Products
(28 results)
