Analysis of a novel bacterial organelle, oligobody in an extremely oligotrophic bacterium
| Project/Area Number |
24580111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka University (2013-2014)
Nara Institute of Science and Technology (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 低栄養性細菌 / オリゴトローフ / ロドコッカス属 / 二酸化炭素 / オリゴボディー / 無機ポリリン酸 / 透過型電子顕微鏡観察 / oligotroph / Rhodococcus erythropolis / oligobody / inorganic polyphosphate / polyphosphate kinase / ポリリン酸 / oligo body / TEM |
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| Outline of Final Research Achievements |
Rhodococcus erythropolis N9T-4 can grow on a minimum salt medium without any additional carbon source and requires CO2 for its oligotrophic growth. This bacterium forms a unique bacterial organelle, oligobody under oligotrophic conditions. The formation of the oligobodies was not affected by additional carbon sources but the size became reduced when NH4Cl was used as a nitrogen source in a dose-dependent manner. Biochemical and TEM-EDX analyses showed that the component of the oligobody was inorganic polyphosphate. Genome analysis of N9T-4 revealed that two genes involved in synthesis and degradation of inorganic polyphosphate, ppk1 and ppk2, respectively. The oligobody formation was dramatically decreased in a ppk1-deletion mutant, while the growth of the mutant was the same level of that of the wild-type strain.
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Report
(4 results)
Research Products
(9 results)
