| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Rhodococcus erythropolis N9T-4 can grow on a minimum salt medium without any additional carbon source and requires CO2 for its oligotrophic growth. This bacterium forms a unique bacterial organelle, oligobody under oligotrophic conditions. The formation of the oligobodies was not affected by additional carbon sources but the size became reduced when NH4Cl was used as a nitrogen source in a dose-dependent manner. Biochemical and TEM-EDX analyses showed that the component of the oligobody was inorganic polyphosphate. Genome analysis of N9T-4 revealed that two genes involved in synthesis and degradation of inorganic polyphosphate, ppk1 and ppk2, respectively. The oligobody formation was dramatically decreased in a ppk1-deletion mutant, while the growth of the mutant was the same level of that of the wild-type strain.
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