Analysis of Agrobacterium strain which has unique characterisitics suitable for rice transformation
Project/Area Number |
24580115
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | Hiroshima University |
Principal Investigator |
SUZUKI Katsunori 広島大学, 理学(系)研究科(研究院), 教授 (50221320)
|
Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Shinji 広島大学, 理学(系)研究科(研究院), 助教 (50607348)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | アグロバクテリア / イネ / 形質転換 / 感染遺伝子発現 / 遺伝子発現誘導物質 / 病原細菌 / 遺伝子発現 / 化学発光レポーター / 遺伝子水平伝達 / 病原性細菌 |
Outline of Final Research Achievements |
The primary aim of this study is to reveal the mechanism of a unique Agrobacterium strain that has property suitable for rice transformation. So far, they thought that rice has no or little material that induces vir gene expression in agrobacteria. Here we successfully extract and purify the substance, and then determined the molecular structure. The fact was supported by an experiment using the chemically synthesized material. This study also showed that the phenotype of the strain is determined not by Ti plasmid but by chromosomal background of the strain.
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Report
(4 results)
Research Products
(13 results)