The mechanism of hyperproduction of citric acid by white koji mold, Aspergillus kawachii and application of production of organic acid
| Project/Area Number |
24580116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
GOTO Masatoshi 九州大学, (連合)農学研究科(研究院), 准教授 (90274521)
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| Co-Investigator(Kenkyū-buntansha) |
FUTAGAMI Taiki 鹿児島大学, 農学部, 准教授 (60512027)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | Aspergillus / 麹菌 / 有機酸発酵 / ゲノム解析 / マイクロアレイ解析 / Aspergilus / 有機酸生産 / クエン酸 / 遺伝子発現 / 応用微生物学 / ゲノム |
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| Outline of Final Research Achievements |
We attempted to elucidate the mechanism for hyperproduction of citric acid by Aspergillus kawachii. A. kawachii ligD-disruptant which exhibits the ability of homologous recombination with high efficiency was constructed and auxotrophic strains were constructed. Approximately 1,100 genes that show differential expression after shifting cultivation at high temperature to cultivation at low temperature were identified. Gene ontology analysis revealed that A. kawachii is stressed from cultivation at high temperature that enhances the amylase activity and responds to protect against heat damage. Cultivation of A. kawachii at low temperature enhances production of citric acid. Under this condition EMP pathway to form pyruvate was reinforced by the repression of metabolic pathways for synthesis of glycerol and trehalose, and pentose phosphate in A. kawachii. Thirty six genes were selected to be important for high production of citric acid and 35 genes were disrupted in A. kawachii.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Transcriptomic analysis of temperature responses of Aspergillus kawachii during barley koji production.2015
Author(s)
Futagami, T., Mori, K., Wada, S., Ida, H., Kajiwara, Y., Takashita, H., Tashiro, K., Yamada, O., Omori, T., Kuhara, S., and Goto, M.
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Journal Title
Appl. Environ. Microbiol.
Volume: 81
Issue: 4
Pages: 1353-1363
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Construction of a <i>ligD</i> disruptant for efficient gene targeting in white koji mold, <i>Aspergillus kawachii </i>2013
Author(s)
Tashiro S, Futagami T, Wada S, Kajiwara Y, Takashita H, Omori T, Takahashi T, Yamada O, Takegawa K, Goto M.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 59
Issue: 3
Pages: 257-260
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed
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[Journal Article] Construction of a ligD disruptant for efficient gene targeting in white koji mold, Aspergillus kawachii.2013
Author(s)
S. Tashiro, T. Futagami, S. Wada, Y. Kajiwara, H. Takashita, T. Omori, T. Takahashi, O. Yamada, K. Takegawa, M. Goto.
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Journal Title
Journal of General and Applied Microbiology
Volume: in press
NAID
Related Report
Peer Reviewed
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[Presentation] Genomic sequence of the shochu koji mold Aspergillus kawachii
Author(s)
T. Futagami, K. Mori, A. Yamashita, S. Wada, Y. Kajiwara, H. Takashita, T. Omori, K. Takegawa, K. Tashiro, S. Kuhara and M. Goto
Organizer
15th International Biotechnology Symposium
Place of Presentation
Daegu, Korea
Related Report
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