Development of one-step gene disruption system of diploid shochu yeast strain using loss of heterozygosity
Project/Area Number |
24580118
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Kagoshima University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | ヘテロ接合性消失 / 焼酎酵母 / 2倍体 / 遺伝子破壊 |
Outline of Final Research Achievements |
It has been thought to be difficult to obtain mutant strain, because shochu yeast is diploid. Thus mutation in one of the two allelic genes is hard to appear as a mutant phenotype. In this study, we constructed a gene disruption system of diploid shochu yeast by using LOH (Loss of Heterozygosity). We designed and constructed a gene disruption cassette by combination of LYS5 a gene involved in Lysine biosynthesis and URA3 a gene involved Uracil biosynthesis. By using this gene disruption cassette, we succeeded in single gene disruption of LEU2 a gene involved in Leucine biosynthesis, and thereafter obtained a double gene disruptant by inducing LOH. Further, we succeeded second round gene disruption after removing the gene disruption cassette from LEU2 double gene disruptant.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Development of a heat-processing method for koji to enhance its antioxidant activity2012
Author(s)
Okutsu, K., Yoshizaki, Y., Takamine, K., Tamaki, H., Ito, K., Sameshima, Y.
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Journal Title
J.Biosci.Bioeng.
Volume: 113(3)
Issue: 3
Pages: 349-354
DOI
NAID
Related Report
Peer Reviewed
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