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Studies on cellulases for the construction of biorefinary from cellulose

Research Project

Project/Area Number 24580120
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionIshikawa Prefectural University

Principal Investigator

KUMAGAI HIDEHIKO  石川県立大学, 生物資源環境学部, 教授 (70027192)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kenji  石川県立大学, 生物資源環境学部, 教授 (70109049)
KATAYAMA Takane  石川県立大学, 寄附講座教授 (70346104)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsバイオリファイナリー / リニューワブル資源 / セルラーゼ / β-グルコシダーゼ / 耐熱性セルラーゼ / 酵素耐熱性向上変異 / 耐熱性酵素発現耐熱性酵母 / β-グルコシダーゼX線構造解析 / β‐グルコシダーゼ / β‐グルコシダーゼX線構造解析 / 未利用資源 / セルロース / 耐熱性 / b-グルコシダーゼ / バイオエタノール / Kluyveromyces / 糖化同時発酵
Outline of Final Research Achievements

We developed ventilation-mediated simultaneous ethanol fermentation and recovery system with a thermo-tolerant yeast strain, K. marxianus. Running the system, apparently pure ethanol (28 g) was recovered from cellobiose (100g) by growing recombinant expressing β-glucosidase from a thermo-tolerant fungus. To create much more thermo-stable enzyme, we mutagenized its gene and obtained five mutant enzymes showing higher thermo-stability than the wild type enzyme. These mutations were integrated in one enzyme molecule and the mutant enzyme showed 10℃ higher Tm value,than that of wild type enzyme. The mutant enzyme showed the same specific activity on its substrates with that of wild type enzyme. The transformant with this mutant enzyme gene showed 1.3times higher productivity of ethanol compared with the strain having wild type β-glucosidase gene. The mutant enzyme was crystallized and the crystalline structure was determined at the 2.7Å resolution.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Remarks (1 results)

  • [Journal Article] Kluyveromyces marxianus-based Platform for Direct Ethanol Fermentation and Recovery from Cellulosic Materials Under Air-Ventilated Condition2012

    • Author(s)
      Matsuzaki, C, Nakagawa, A., Koyanagi, T., Tamaki, H., et al
    • Journal Title

      J.Biosci.Bioeng.

      Volume: 113 Issue: 5 Pages: 604-607

    • DOI

      10.1016/j.jbiosc.2011.12.007

    • NAID

      110009456899

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Remarks] Kluyveromyces marxianus-based platform for ・・・・

    • URL

      https://reginnovations.org/?s=matsuzaki

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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