Investigation of a novel transcriptional repression mechanism of PCB degradation genes
| Project/Area Number |
24580121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 転写制御 / カタボライト抑制 / 細菌 / Rhodococcus / ポリ塩化ビフェニル / PCB / バクテリア / 芳香族分解 |
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| Outline of Final Research Achievements |
In Rhodococcus jostii RHA1, known as a polychlorinated biphenyl (PCB) degrader, the transcription of biphenyl degradation genes is up-regulated in the presence of biphenyl, while it is repressed by the coexistence of benzoate which is one of the intermediate compounds of biphenyl degradation. In this study, the mechanism of this repression was investigated. It was revealed that it is catechol, a downstream compound of benzoate degradation, that causes the repression of the transcription of biphenyl degradation genes, and the overexpression of the catechol degradation gene (catA) in RHA1 canceled the repression and enhanced the growth of RHA1 on biphenyl. Genes which have been known to be responsible for the catabolite repression, crp, was proved not to participate in this repression.
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Report
(4 results)
Research Products
(6 results)
