Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
Deep-sea piezophiles have adapted to high-pressure environments. The cell membrane is important for substrate transports and energy production, and its functions are generally sensitive to high pressure. This study investigates the microbial adaptation to high pressurein terms of membrane dynamics. Deep-sea isolates of Shewanella, Moritella and Photobacterim were subjected to time-resolved anisotropy measurement using TMA-DPH as a polarization probe to measure the membrane order and the rotational motion of the acyl chains. I found that bacterial strains isoalted from deeper habitats had more rigid cell membrane. This view is distinct from common belief that deep-sea microbes have more fluid cell membrane.
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