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Piezo-adaptation of deep-sea microbes analyzed by measurements of the membrane dynamics

Research Project

Project/Area Number 24580122
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionAoyama Gakuin University

Principal Investigator

ABE Fumiyoshi  青山学院大学, 理工学部, 教授 (30360746)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords深海好圧性細菌 / 細胞膜 / 膜物性 / 時間分解蛍光偏光解消法 / 高圧適応 / 深海性好圧性細菌 / 細胞膜物性 / TMA-DPH / 至適増殖圧力 / 圧力適応 / 深海微生物 / 細胞膜ダイナミクス
Outline of Final Research Achievements

Deep-sea piezophiles have adapted to high-pressure environments. The cell membrane is important for substrate transports and energy production, and its functions are generally sensitive to high pressure. This study investigates the microbial adaptation to high pressurein terms of membrane dynamics. Deep-sea isolates of Shewanella, Moritella and Photobacterim were subjected to time-resolved anisotropy measurement using TMA-DPH as a polarization probe to measure the membrane order and the rotational motion of the acyl chains. I found that bacterial strains isoalted from deeper habitats had more rigid cell membrane. This view is distinct from common belief that deep-sea microbes have more fluid cell membrane.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (8 results) (of which Invited: 3 results) Book (2 results)

  • [Journal Article] Effects of high hydrostatic pressure on microbial cell membranes: Structural and functional perspectives2015

    • Author(s)
      Abe, F.
    • Journal Title

      High Pressure Bioscience -Basic Concepts, Applications and Frontiers, Springer Subcellular Biochemistry (SBM)

      Volume: なし

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Stress responses of the yeast Saccharomyces cerevisiae under high hydrostatic pressure2015

    • Author(s)
      Abe, F.
    • Journal Title

      Stress Biology of Yeasts and Fungi: Application for Industrial Brewing and Fermentation, Springer

      Volume: なし Pages: 77-92

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Functional implications and ubiquitin-dependent degradation of the peptide transporter Ptr2 in Saccharomyces cerevisiae2014

    • Author(s)
      Kawai, K., Moriya, A., Uemura, S. and Abe, F.
    • Journal Title

      Eukaryotic Cell

      Volume: 13 Issue: 11 Pages: 1380-1392

    • DOI

      10.1128/ec.00094-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional mapping and implications of substrate specificity of the yeast high-affinity leucine permease Bap22014

    • Author(s)
      上村 聡志,井ノ口 仁一 他
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1838 Issue: 7 Pages: 1719-1729

    • DOI

      10.1016/j.bbamem.2014.03.018

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural and functional implications of the yeast high-affinity tryptophan permease Tat22013

    • Author(s)
      Kanda, N. and Abe, F.
    • Journal Title

      Biochemistry

      Volume: 52 Issue: 25 Pages: 4296-4307

    • DOI

      10.1021/bi4004638

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pressure-induced endocytic degradation of the yeast low-affinity tryptophan permease Tat1 is mediated by Rsp5 ubiquitin ligase and functionally redundant PPxY-motif proteins2013

    • Author(s)
      Suzuki, A., Mochizuki, T., Uemura, S., Hiraki, T. and Abe, F.
    • Journal Title

      Eukaryotic Cell

      Volume: 12 Issue: 7 Pages: 990-997

    • DOI

      10.1128/ec.00049-13

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of high hydrostatic pressure on the dynamic structure of living Escherichia coli membrane: a study using high-pressure time-resolved fluorescence anisotropy measurement2013

    • Author(s)
      Abe, F. and Usui, K.
    • Journal Title

      High Pressure Research

      Volume: 33 Issue: 2 Pages: 278-284

    • DOI

      10.1080/08957959.2013.769533

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of high hydrostatic pressure on the dynamic structure of living Escherichia coli membrane: a study using high-pressure time-resolved fluorescence anisotropy measurement2013

    • Author(s)
      Abe, F. and Usui, K
    • Journal Title

      High Pressure Research

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamic structural changes in microbial membranes in response to high hydrostatic pressure analyzed using time-resolved fluorescence anisotropy measurement2013

    • Author(s)
      Abe, F.
    • Journal Title

      Biophys. Chem.

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 好圧性細菌における膜物性の直接計測 ー高圧蛍光偏光解消法からのアプローチー2012

    • Author(s)
      阿部文快
    • Journal Title

      J. Jpn. Soc. Extremophiles

      Volume: 11 Pages: 83-89

    • NAID

      40019593236

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ユビキチン化による出芽酵母のアミノ酸・ペプチド輸送体の品質管理2015

    • Author(s)
      阿部文快
    • Organizer
      日本農芸化学会2015年大会シンポジウム
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 出芽酵母のロイシン輸送体Bap2における基質認識2014

    • Author(s)
      宇佐美佑紀、上村聡志、望月貴博、阿部文快
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      東京大学
    • Year and Date
      2014-09-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of growth-permissive pressures on the physiology of yeast2014

    • Author(s)
      Abe, F.
    • Organizer
      8th International Conference on High Pressure Bioscience and Biotechnology 2014 (HPBB2014)
    • Place of Presentation
      フランス、ナント
    • Year and Date
      2014-07-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Effects of growth-permissive pressures on the physiology of yeast2014

    • Author(s)
      Fumiyoshi Abe
    • Organizer
      8th International Conference on High Pressure Bioscience and Biotechnology 2014
    • Place of Presentation
      Nantes, France
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 出芽酵母のロイシン輸送体Bap2における機能部位の同定

    • Author(s)
      宇佐美佑紀、望月貴博、上村聡志、阿部文快
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 高温・高圧ストレス下における酵母トリプトファン輸送体Tat1の安定性に関する解析

    • Author(s)
      望月貴博、持田和範、上村聡志、阿部文快
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 出芽酵母のペプチド輸送体Ptr2の発現と機能解析

    • Author(s)
      河合建、上村聡志、阿部文快
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Research-status Report
  • [Presentation] トリプトファン輸送体Tat2における基質認識とプロトン共輸送モデル

    • Author(s)
      阿部文快、完田奈緒子
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      東北学院大学
    • Related Report
      2013 Research-status Report
  • [Book] 高圧力下における微生物細胞膜の動的構造2013

    • Author(s)
      阿部文快
    • Publisher
      高圧力の科学と技術
    • Related Report
      2012 Research-status Report
  • [Book] 出芽酵母における非致死的圧力への応答と適応 ~ゲノムからのアプローチ2013

    • Author(s)
      阿部文快
    • Publisher
      エヌ・ティー・エス出版
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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