Piezo-adaptation of deep-sea microbes analyzed by measurements of the membrane dynamics
| Project/Area Number |
24580122
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Aoyama Gakuin University |
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Principal Investigator |
ABE Fumiyoshi 青山学院大学, 理工学部, 教授 (30360746)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 深海好圧性細菌 / 細胞膜 / 膜物性 / 時間分解蛍光偏光解消法 / 高圧適応 / 深海性好圧性細菌 / 細胞膜物性 / TMA-DPH / 至適増殖圧力 / 圧力適応 / 深海微生物 / 細胞膜ダイナミクス |
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| Outline of Final Research Achievements |
Deep-sea piezophiles have adapted to high-pressure environments. The cell membrane is important for substrate transports and energy production, and its functions are generally sensitive to high pressure. This study investigates the microbial adaptation to high pressurein terms of membrane dynamics. Deep-sea isolates of Shewanella, Moritella and Photobacterim were subjected to time-resolved anisotropy measurement using TMA-DPH as a polarization probe to measure the membrane order and the rotational motion of the acyl chains. I found that bacterial strains isoalted from deeper habitats had more rigid cell membrane. This view is distinct from common belief that deep-sea microbes have more fluid cell membrane.
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Report
(4 results)
Research Products
(20 results)
