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Regulation of two-component regulatory system genes by active protein degradation of transcription factor

Research Project

Project/Area Number 24580123
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied microbiology
Research InstitutionTokai University

Principal Investigator

OGURA Mitsuo  東海大学, 海洋研究所, 教授 (80204163)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsタンパク質分解 / ATP依存性プロテアーゼ / 遺伝子発現 / DNA結合 / グルコース誘導 / 転写因子 / グルコース応答 / ECF シグマ因子 / DegS-DegU
Outline of Final Research Achievements

The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU are composed of an operon directed by the P1 promoter.
Spx regulates diamide-stress regulon in addition to many unknown genes and is a substrate of the ATP-dependent ClpXP protease. We have found that null mutations for clpX and clpP, which encode subunits for ClpXP, enhanced the DegU amount through activation of the P1 promoter. We isolated four suppressors for the clpP-enhancing effect. Whole-genome sequencing of the suppressors revealed that the strains have a point mutation or deletion in spx. The clpP-enhancing effect on degS-lacZ expression was abolished in the spx disruptant. These results showed that the degSU operon is a new target of positive regulation by Spx. Furthermore, we found that the P1 promoter was induced by glucose and that this induction was greatly reduced in the spx mutant. These results suggested that Spx is involved in glucose induction.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) Remarks (2 results)

  • [Journal Article] Bacillus subtilis degSU operon is regulated by the ClpXP-Spx regulated proteolysis system.2015

    • Author(s)
      Shiwa Y, Yoshikawa H, Tanaka T, Ogura M.
    • Journal Title

      J. Biochem

      Volume: 157 Issue: 5 Pages: 321-330

    • DOI

      10.1093/jb/mvu076

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Regulation of the response regulator gene degU through the binding of SinR/SlrR and exclusion of SinR/SlrR by DegU in Bacillus subtilis.2014

    • Author(s)
      Ogura M, Yoshikawa H, Chibazakura T.
    • Journal Title

      J Bacteriol.

      Volume: 196 Issue: 4 Pages: 873-881

    • DOI

      10.1128/jb.01321-13

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Bacillus subtilis response regulator gene degU is positively regulated by CcpA and by catabolite-repressed synthesis of ClpC.2013

    • Author(s)
      Ishii, H., Tanaka, T., and Ogura, M.
    • Journal Title

      Journal of Bacteriology

      Volume: 195 Issue: 2 Pages: 193-201

    • DOI

      10.1128/jb.01881-12

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] SwrA regulates assembly of Bacillus subtilis DegU via its interaction with N-terminal domain of DegU.2012

    • Author(s)
      Ogura, M., and Tsukahara, K.
    • Journal Title

      Journal of Biochemistry

      Volume: 151 Issue: 6 Pages: 643-655

    • DOI

      10.1093/jb/mvs036

    • NAID

      10031166106

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Post-transcriptional regulation of SinI/SinR/SlrR by DegU in Bacillus subtilis.2015

    • Author(s)
      安部公博、佐藤勉、小倉光雄
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学百年記念講堂(兵庫県)
    • Year and Date
      2015-03-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Post-transcriptional regulation of SinI/SinR/SlrR biofilm regulation system by DegU in Bacillus subtilis.2014

    • Author(s)
      小倉光雄、佐藤勉、安部公博
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] RNAPアセチル化は、RNAPのECFシグマ因子選好性を制御する I: sigX/M/(I)発現のアンチシグマ非依存的なグルコース誘導2014

    • Author(s)
      小倉光雄、田中暉夫、朝井計、志波優、吉川博文
    • Organizer
      平成26年度 グラム陽性菌ゲノム機能会議
    • Place of Presentation
      庄内いこいの村(山形県)
    • Year and Date
      2014-09-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] RNAPアセチル化は、RNAPのECFシグマ因子選好性を制御するII: アセチル化リジン変異によるSigXのグルコース誘導消失2014

    • Author(s)
      古園さおり、米沢祐大、鈴木祥太、朝井計、小倉光雄
    • Organizer
      平成26年度 グラム陽性菌ゲノム機能会議
    • Place of Presentation
      庄内いこいの村(山形県)
    • Year and Date
      2014-09-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 枯草菌Response regulator DegUの遺伝子発現は、SinR/SlrR複合体の結合とDegUによるSinR/SlrRの排除により制御されている2014

    • Author(s)
      小倉 光雄, 吉川博文, 千葉櫻拓
    • Organizer
      第8回日本ゲノム微生物学会
    • Place of Presentation
      東京農業大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 枯草菌の転写因子DegUは、エピジェネティック複合体の構成因子SlrRとタンパク質相互作用し、そのコロニー形態を制御している2013

    • Author(s)
      小倉 光雄、 石井 洋、 吉川博文、 千葉櫻拓
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 枯草菌degU遺伝子発現のCcpAによる制御

    • Author(s)
      石井 洋、田中暉夫、 小倉 光雄
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ
    • Related Report
      2012 Research-status Report
  • [Remarks] 東海大学海洋研究所

    • URL

      http://www.scc.u-tokai.ac.jp/~289077/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東海大学海洋研究所・生物センター

    • URL

      http://www.iord.u-tokai.ac.jp/staff/ogura-jp.htm

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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