| Project/Area Number |
24580124
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo Denki University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HASHIMOTO Ken-ichi 東京電機大学, 工学部, 助教 (70508382)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 排出 / 代謝工学 / 合成生物学 / 発酵 / Corynebacterium / チャネル / パッチクランプ法 / 応用微生物 / 電気生理 |
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| Outline of Final Research Achievements |
While it is hoped that microbial cell factories can be created to produce various useful compounds, it is thought unlikely that a pre-existing natural exporter exists that can selectively export all of these useful compounds out of the cell. In addition, rational design of such exporters is, at present, extremely difficult. This is thought to be one cause of the inability of designed microbial cell factories to perform as expected, and there is demand for a breakthrough for this problem.
In this study, having confirmed the export activity of actual gene product using our unique path-clamp system, we introduced exporter genes into microbial cell factories, and showed that exporter engineering is highly important in the creation of microbial cell factories by systems biology or synthetic biology.
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