Uncovering the soil microbial community structure based on humus-metaboilzing activity of actinomycetes

• Project/Area Number: 24580128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Nihon University
• Principal Investigator: UEDA Kenji 日本大学, 生物資源科学部, 教授 (00277401)
• Co-Investigator(Kenkyū-buntansha): TAKANO Hideaki 日本大学, 生物資源科学部, 助教 (50385994)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: Streptomyces / humic acid / respiration / electron transfer / differentiation / secondary metabolism / humus

Outline of Final Research Achievements

This study aimed at elucidating how the soil-dwelling gram-positive bacteria actinomycetes contributes to the construction of microbial community structure. Some actinomycetes strains exhibited a marked humus-coagulating and decolorizing activity． The isolation of humus-decolorizing activity of Streptomyces coelicolor A3(2) showed the possible involvement of a membrane-associating diaphorase． The presence of similar extracytoplasmic diaphorase activity in the diverged actinomycetes strains suggested the wide occurrence of energy metabolism based on the reduction of a humic substance(s)． Overall，the evidence suggests that some actinomycetes metabolites widely support the growth and expression of specific functions in other organisms，and that actinomycetes contributes to the construction of a global soil microbial community structure by effectively associating with the formation and dynamics of humus，the substance derived from dead plant that constitutes rich soil environment.

Research Products

• [Journal Article] Fundamental role of cobalamin biosynthesis in the developmental growth of Streptomyces coelicolor A3 (2) (2015)
  • Author(s): H Takano, K Hagiwara, K Ueda
  • Journal Title: Appl Microbiol Biotechnol Volume: 99 Issue: 5 Pages: 2329-2337
  • DOI: 10.1007/s00253-014-6325-z
  • Peer Reviewed
• [Journal Article] Amide-transforming activity of Streptomyces: possible application to the formation of hydroxy amides and aminoalcohols. (2013)
  • Author(s): Yamada S, Miyagawa TA, Yamada R, Shiratori-Takano H, Sayo N, Saito T, Takano H, Beppu T, Ueda K.
  • Journal Title: Appl Microbiol Biotechnol Volume: 97 Issue: 14 Pages: 6223-6230
  • DOI: 10.1007/s00253-013-4952-4
  • Peer Reviewed
• [Journal Article] Divergent effects of desferrioxamine on bacterial growth and characteristics. (2013)
  • Author(s): Eto D, Watanabe K, Saeki H, Oinuma KI, Otani KI, Nobukuni M, Shiratori-Takano H, Takano H, Beppu T, Ueda K.
  • Journal Title: J Antibiot Volume: 2012 Issue: 4 Pages: 1-5
  • DOI: 10.1038/ja.2012.111
  • NAID: 10031168679
  • Peer Reviewed
• [Presentation] Streptomyces属放線菌が産生する菌体外ジアホラーゼの解析 (2015)
  • Author(s): 老沼研一、進藤大騎、髙野英晃、高谷直樹、上田賢志
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学（岡山県岡山市）
  • Year and Date: 2015-03-29
• [Presentation] ATP synthesis inhibitor at subinhibitory concentration (2015)
  • Author(s): K. Ueda
  • Organizer: Natural Product - Discovery & Development in The Post Genomic Era
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2015-01-11 – 2015-01-14
  • Invited
• [Presentation] ストレプトミセス属放線菌のエネルギー代謝と分化の連携 (2013)
  • Author(s): 藤本正浩、高野英晃、上田賢志
  • Organizer: 日本農芸化学会
  • Place of Presentation: 仙台
  • Invited