DNA rearrangement in spore-forming bacteria
Project/Area Number |
24580130
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Hosei University |
Principal Investigator |
SATO Tsutomu 法政大学, 生命科学部, 教授 (70215812)
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 細菌 / ファージ / DNA再編成 / 細胞分化 / 枯草菌 / 遺伝子発現 / 遺伝子発見 |
Outline of Final Research Achievements |
Integration of prophages into protein-coding sequences of the host chromosome generally results in loss of function of the interrupted gene. We have shown that several phage (-like) elements are inserted into mother cell-specific sporulation genes in many spore-forming bacteria. Remarkably, functional sporulation genes are reconstituted during sporulation by limiting prophage excision to the mother cell genome. We have also reported that an active prophage, SPβ, is inserted into a polysaccharide synthesis gene, spsM, which is expressed in the mother cell during sporulation in B. subtilis. SPβ excision can occur either during the phage lytic cycle or during sporulation to reconstitute a functional spsM gene. Our results suggest that this type of gene reconstitution is a common mechanism in spore-forming bacteria.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] 枯草菌胞子最外層の役割2014
Author(s)
岩本敬人, 稲井貴志, 井之口紫苑, 安部公博, 佐藤勉
Organizer
グラム陽性菌ゲノム機能会議
Place of Presentation
いこいの村庄内(山形県・鶴岡市)
Year and Date
2014-09-04
Related Report
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[Presentation] 枯草菌胞子最外層の役割2014
Author(s)
岩本敬人,稲井貴志, 井之口紫苑, 安部公博, 佐藤勉
Organizer
微生物研究会
Place of Presentation
東京農業大学(東京都世田谷区)
Year and Date
2014-07-26
Related Report
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