| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Integration of prophages into protein-coding sequences of the host chromosome generally results in loss of function of the interrupted gene. We have shown that several phage (-like) elements are inserted into mother cell-specific sporulation genes in many spore-forming bacteria. Remarkably, functional sporulation genes are reconstituted during sporulation by limiting prophage excision to the mother cell genome. We have also reported that an active prophage, SPβ, is inserted into a polysaccharide synthesis gene, spsM, which is expressed in the mother cell during sporulation in B. subtilis. SPβ excision can occur either during the phage lytic cycle or during sporulation to reconstitute a functional spsM gene. Our results suggest that this type of gene reconstitution is a common mechanism in spore-forming bacteria.
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