Biochemical and genetic analysis of the methylation-promoting factor for the RsmG methyltransferase
| Project/Area Number |
24580131
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
OKAMOTO Susumu 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所食品バイオテクノロジー研究領域, 上席研究員 (80353986)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | RNA / 塩基修飾 / メチル化酵素 / 塩基就職 / 微生物 |
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| Outline of Final Research Achievements |
Bacterial rRNAs have many modified nucleotides. Among them, a 7-methylguanosine (m7G) modification in 16S rRNA is highly conserved among eubacteria. This m7G modification is catalyzed by the RsmG methyltransferase. In Escherichia coli, the methylation reaction catalyzed by RsmG is significantly promoted by an unknown protein factor. Here, we conducted biochemical and genetic analyses of this methylation-promoting factor. We have identified the Tas protein as a candidate.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins.2015
Author(s)
Lee K, Kusumoto M, Sekizuka T, Kuroda M, Uchida I, Iwata T, Okamoto S, Yabe K, Inaoka T, Akiba M
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Journal Title
Front Microbiol
Volume: 6
Pages: 78-78
DOI
Related Report
Peer Reviewed / Open Access
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