Biochemical and genetic analysis of the methylation-promoting factor for the RsmG methyltransferase

Research Project

Project/Area Number	24580131
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Applied microbiology
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	OKAMOTO Susumu 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所食品バイオテクノロジー研究領域, 上席研究員 (80353986)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	RNA / 塩基修飾 / メチル化酵素 / 塩基就職 / 微生物
Outline of Final Research Achievements	Bacterial rRNAs have many modified nucleotides. Among them, a 7-methylguanosine (m7G) modification in 16S rRNA is highly conserved among eubacteria. This m7G modification is catalyzed by the RsmG methyltransferase. In Escherichia coli, the methylation reaction catalyzed by RsmG is significantly promoted by an unknown protein factor. Here, we conducted biochemical and genetic analyses of this methylation-promoting factor. We have identified the Tas protein as a candidate.

Report

(4 results)