Identification and functional analysis of novel metabolizing enzymes of plant hormones
Project/Area Number |
24580134
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Hokkaido University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SABURI Wataru 北海道大学, 大学院農学研究院, 助教 (00598089)
MATSUURA Hideyuki 北海道大学, 大学院農学研究院, 准教授 (20344492)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | ジャスモン酸 / シトクロムP450 / ツベロン酸 / 植物ホルモン / CYP / シロイヌナズナ / 水酸化 / モノオキシゲナーゼ / P450 |
Outline of Final Research Achievements |
Jasmonate (JA) is a plant hormone regulating abiotic and biotic stress responses and growth. In this study, we analyzed the physiological and biochemical functions of CYP94D1, CYP94D2, and ILL6 proteins, which were predicted to inactivate the JA signal. In the analysis of mutant plants with higher and lower expression levels of CYP94D1 than wild type plant, we observed phenotypic differences. The transgenic plants lucking CYP94D1 showed higher resistance to external JA. The overexpressing plants of CYP94D1 accumulate higher level of tuberonic acid glucoside than wild type.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Modulation of allosteric regulation by E38K and G101N mutations in the potate tuber ADP-glucose pyrophosphorylase2013
Author(s)
Shinji Wakuta, Yumi Shibata, Yumiko Yoshizaki, Wataru Saburi, Shigeki Hamada, Hiroyuki Ito, Seon-Kap Hwang, Thomas W. Okita, and Hirokazu Matsui
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Pages: 1854-1859
Related Report
Peer Reviewed
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[Journal Article] Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, β-1,4-mannooligosaccharide phosphorylase2012
Author(s)
Ryosuke Kawahara, Wataru Saburi, Rei Odaka, Hidenori Taguchi, Shigeaki Ito, Haruhide Mori, and Hirokazu Matsui
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Journal Title
Journal of Biological Chemistry
Volume: 287
Issue: 50
Pages: 42389-42399
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Purification and characterization of a liuefying α-amylase from alkalophilic termophilic Bacillus sp. AAH-312012
Author(s)
Daehoon Kim, Naoki Morimoto, Wataru Saburi, Atsushi Mukai, Koji Imoto, Toshihiko Takehana, Seiji Koike, Haruhide Mori, Hirokazu Matsui
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Pages: 1378-1383
Related Report
Peer Reviewed
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