Characterization of novel yeast transporter and improvement of method for measurement of organelle ion transporter
| Project/Area Number |
24580135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
Hamamoto Shin 東北大学, 工学(系)研究科(研究院), 助教 (10533812)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | イオンチャネル / 酵母 / 液胞膜 / 細胞内小器官 / パッチクランプ / 酵母液胞膜 / 電気生理学 / 液胞 / オルガネラ膜 |
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| Outline of Final Research Achievements |
YVC1 is a major cation channel localized in yeast vacuole membrane, and it is activated by cytoplasmic Ca2+. We have applied the patch-clamp technique using enlarged yeast cells to elucidate the regulatory mechanisms of the YVC1-mediated channel activity. We have tested the addition of reducing agent into the buffer corresponding to the cytosolic side. Moreover, we have identified the cysteine crucial for the functional property of the YVC1 channel in the presence of thiol group chemicals. And also we measured the single-channel conductance of both of the wild-type YVC1 channel and serine substituted YVC1 channel. The both channels have consistent conductance 300 pS, which suggested that substitution to serine did not affect to the channel conductance. We examined the effect of inositol phosphate on TPC2 channel expressed in yeast vacuole membrane.
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis2015
Author(s)
H. Saito, T. Oikawa, S. Hamamoto, Y. Ishimaru, T. Utsumi, J. Chen, M. Kanamori, Y. Sasaki-Sekimoto, M. Shimojima, S. Masuda, Y. Kamiya, M. Seo, N. Uozumi, M. Ueda, H. Ohta, A
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Journal Title
Nature Communications
Volume: 6
Issue: 1
Pages: 6095-6095
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The Phosphoinositide PI(3,5)P2 mediates Activation of Mammalian but not Plant TPC Proteins: Functional Expression of Endolysosomal Channels in Yeast and Plant Cells2014
Author(s)
Anna Boccaccio, Joachim Scholz-Starke, Shin Hamamoto, Nina Larisch, Margherita Festa, Paul Vijay Kanth Gutla, Alex Costa, Petra Dietrich, Nobuyuki Uozumi, Armando Carpaneto
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Journal Title
Cellular and Molecular Life Sciences
Volume: 掲載確定
Related Report
Peer Reviewed
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[Journal Article] Defining membrane spanning domains and crucial membrane-localized acidic amino acid residues for K + transport of a Kup/HAK/KT-type Escherichia coli potassium transporter.2014
Author(s)
Sato, Y., Nanatani, K., Hamamoto, S., Shimizu, M., Takahashi, M., Tabuchi-Kobayashi, M., Mizutani, A., Schroeder, J.I.,Souma, S, and Uozumi, N.
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Journal Title
The Journal of Biochemistry
Volume: 掲載確定
Issue: 5
Pages: 315-323
DOI
NAID
Related Report
Peer Reviewed
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