| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Molecular partner of SraA, which was supposed to be a sensor of amino acid, was searched and we identified TrpD, a second enzyme on biosynthesis of tryptophan. We performed biochemical analyses and suggested that SraA sensed tryptophan, which was an end product of the biosynthetic pathway, and transmitted the signal toward TrpD to inhibit the activity. This is a novel mechanism for feedback inhibition of tryptophan biosynthetic pathway. We also found GDH interacted with a homolog of enzyme involved in purine biosynthetic pathway and indicated existence of a novel regulatory mechanism of intracellular metabolism.
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