| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we focused on calcium-binding proteins that are recruited to the specialized region of endoplasmic reticulum, called ER exit site (ERES) and investigated their roles in the early secretory pathway in response to calcium oscillation. The penta-EF-hand protein ALG-2 is a calcium-dependent interacting protein for Sec31A, an outer coat component of COPII, and recruits a calcium-dependent phospholipid-binding protein annexin A11 (AnxA11) to the ERES. The siRNA-mediated knockdown experiments revealed that both ALG-2 and AnxA11 stabilize Sec31A at the ERES and regulate intracellular distribution of the ERES. In the knockdown cells, the transport of GFP-fused vesicular stomatitis virus tsO45 glycoprotein from the ER to the Golgi was accelerated. These results suggest that AnxA11 regulates architectural and functional features of the ERES by coordinating with ALG-2 in response to calcium oscillation.
|
