Analysis of oscillatory calcium signaling and its decoding machinery in the early secretory pathway
| Project/Area Number |
24580138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
SHIBATA Hideki 名古屋大学, 生命農学研究科, 准教授 (30314470)
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| Co-Investigator(Renkei-kenkyūsha) |
MAKI Masatoshi 名古屋大学, 大学院生命農学研究科, 教授 (40183610)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | シグナル伝達 / カルシウム結合タンパク質 / COPII小胞 / 小胞体 / アネキシン |
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| Outline of Final Research Achievements |
In this study, we focused on calcium-binding proteins that are recruited to the specialized region of endoplasmic reticulum, called ER exit site (ERES) and investigated their roles in the early secretory pathway in response to calcium oscillation. The penta-EF-hand protein ALG-2 is a calcium-dependent interacting protein for Sec31A, an outer coat component of COPII, and recruits a calcium-dependent phospholipid-binding protein annexin A11 (AnxA11) to the ERES. The siRNA-mediated knockdown experiments revealed that both ALG-2 and AnxA11 stabilize Sec31A at the ERES and regulate intracellular distribution of the ERES. In the knockdown cells, the transport of GFP-fused vesicular stomatitis virus tsO45 glycoprotein from the ER to the Golgi was accelerated. These results suggest that AnxA11 regulates architectural and functional features of the ERES by coordinating with ALG-2 in response to calcium oscillation.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Structural Analysis of the Complex between Penta-EF-Hand ALG-2 Protein and Sec31A Peptide Reveals a Novel Target Recognition Mechanism of ALG-22015
Author(s)
Takeshi Takahashi, Kyosuke Kojima, Wei Zhang, Kanae Sasaki, Masaru Ito, Hironori Suzuki, Masato Kawasaki, Soichi Wakatsuki, Terunao Takahara, Hideki Shibata, Masatoshi Maki
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Journal Title
International Journal of Molecular Sciences
Volume: 16
Issue: 2
Pages: 3677-3699
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Potential role of annexin A11 in the COPII biogenesis in SH-SY5Y human neuroblastoma cells.2013
Author(s)
Hideki Shibata, Takashi Kanadome, Satoshi Kamura, Akane Sato, Terunao Takahara, Stephen E. Moss, Masatoshi Maki
Organizer
7th International Conference on ANNEXINS
Place of Presentation
The Chaterhouse Square campus of the St Barts and the London School of Medicine and Dentistry, Queen Mary, University of London, London, United Kingdom
Related Report
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