Elucidation of post-translational regulation of ABCG proteiins

• Project/Area Number: 24580139
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto Women's University (2014-2015); Kyoto University (2012-2013)
• Principal Investigator: MATSUO MICHINORI 京都女子大学, 家政学部, 准教授 (00335308)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ABCタンパク質 / コレステロール / モジュレーター / 脂質 / トランスポーター / メタボリックシンドローム / 動脈硬化 / アルツハイマー病 / プロテインキナーゼ

Outline of Final Research Achievements

The aim of this study is to elucidate lipid homeostasis in our body by analyzing post-translational regulation of ABCG proteins involved in lipid efflux from cells. Regulation mechanism of ABCG1 and ABCG4 in neuronal cells and functional regulation of ABCG proteins by fatty acids and interacting proteins were demonstrated. The results may lead to prevention and treatment of metabolic syndrome and Alzheimer’s disease.

Research Products

• [Journal Article] Possible application of apolipoprotein E-containing lipoproteins and polyunsaturated fatty acids in neural regeneration. (2016)
  • Author(s): Matsuo, M.
  • Journal Title: Neural Regene. Res. Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ABCG1 and ABCG4 suppress γ-secretase activity and amyloid β production. (2016)
  • Author(s): Sano, O., Tsujita, M., Shimizu, Y., Kato, R., Kobayashi, A., Kioka, N., Ramaley, A.T.., Michikawa, M., Ueda K., and Matsuo, M.
  • Journal Title: PLoS ONE Volume: 印刷中
  • NAID: 120005997762
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Neurite outgrowth stimulation by n3 and n6 polyunsaturated fatty acids of phospholipids in apolipoprotein E containing lipoproteins secreted from glial cells. (2015)
  • Author(s): Nakato M, Matsuo M, Kono N, Arita M, Arai H, Ogawa J, Kioka N, Ueda K.
  • Journal Title: J Lipid Res Volume: 56 Issue: 10 Pages: 1880-1890
  • DOI: 10.1194/jlr.m058164
  • Peer Reviewed / Open Access
• [Journal Article] Position 834 in TM6 plays an important role in cholesterol and phosphatidylcholine transport by ABCA1 (2015)
  • Author(s): Shimpei Ito, Kohjiro Nagao, Yasuhisa Kimura, Michinori Matsuo, Noriyuki Kioka, Kazumitsu Ueda
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 79 Issue: 5 Pages: 775-781
  • DOI: 10.1080/09168451.2014.993358
  • Peer Reviewed
• [Journal Article] ABCB4 exports phosphatidylcholine in a sphingomyelin-dependent manner (2015)
  • Author(s): Zhao, Y., Ishigami, M., Nagao, K., Hanada, K., Kono, N., Arai, H., Matsuo, M., Kioka, N., and Ueda, K.
  • Journal Title: J. Lipid Res. Volume: 56 Issue: 3 Pages: 644-652
  • DOI: 10.1194/jlr.m056622
  • Peer Reviewed
• [Journal Article] Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation (2014)
  • Author(s): Tomiyama, L., Sezaki, T., Matsuo, M., Ueda, K. and Kioka, N.
  • Journal Title: Oncogene Volume: 33 Issue: 9 Pages: 1141-1149
  • DOI: 10.1038/onc.2014.31
  • Peer Reviewed
• [Journal Article] ABCA1, ABCG1, and ABCG4 are distributed to distinct membrane meso-domains and disturb detergent-resistant domains on the plasma membrane (2014)
  • Author(s): Sano, O., Ito, S., Kato, R., Shimizu, Y., Kobayashi, A., Kimura, Y., Kioka, N., Hanada, K., Ueda K., and Matsuo, M.
  • Journal Title: PLoS ONE Volume: 9 Issue: 10 Pages: e109886-e109886
  • DOI: 10.1371/journal.pone.0109886
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fomiroid A, a novel compound from the mushroom Fomitopsis nigra, inhibits NPC1L1-mediated cholesterol uptake via a mode of action distinct from that of ezetimibe (2014)
  • Author(s): Chiba, T., Sakurada, T., Watanabe, R., Yamaguchi, K., Kimura, Y. Kioka, N., Kawagishi, H., Matsuo, M., and Ueda K
  • Journal Title: PLoS ONE Volume: 9 Issue: 12 Pages: e116162-e116162
  • DOI: 10.1371/journal.pone.0116162
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 24(S)-hydroxycholesterol is actively eliminated from neuronal cells by ABCA1. (2013)
  • Author(s): Matsuda, A., Nagao, K, Matsuo, M., Kioka N. and Ueda, K.
  • Journal Title: J. Neurochem. Volume: (in press) Issue: 1 Pages: 93-101
  • DOI: 10.1111/jnc.12275
  • Peer Reviewed
• [Journal Article] ATPase activity of nucleotide binding domains of human MDR3 in the context of MDR1 (2013)
  • Author(s): Ishigami, M.. Tominaga, Y., Nagao, K., Kimura, Y., Matsuo, M., Kioka, N. and Ueda, K
  • Journal Title: Biochim Biophys Acta-MCBL Volume: 1831 Issue: 4 Pages: 683-690
  • DOI: 10.1016/j.bbalip.2012.12.016
  • Peer Reviewed
• [Journal Article] ATPase activity of human ABCG1 is stimulated by cholesterol and sphingomyelin. (2013)
  • Author(s): Hirayama, H., Kimura, Y., Kioka, N., Matsuo,M. and Ueda, K.
  • Journal Title: J. Lipid Res. Volume: 54 Issue: 2 Pages: 496-502
  • DOI: 10.1194/jlr.m033209
  • Peer Reviewed
• [Journal Article] Role of Dlg5/1p-dlg, a membrane-associated guanylate kinase family protein, in epithelial-mesenchymal transition in LLc-PK1 renal epithelial cells (2012)
  • Author(s): Takuhito Sezaki, Kohki Inada, Takayuki Sogabe, Kumiyo Kakuda, Lucia Tomiyama, Yohsuke Matsuno, Takafumi Ichikawa, Michinori Matsuo, Kazumitsu Ueda, Noriyuki Kioka
  • Journal Title: PLoS ONE Volume: 7 Issue: 4 Pages: e35519-e35519
  • DOI: 10.1371/journal.pone.0035519
  • Peer Reviewed
• [Journal Article] ATP hydrolysis-dependent conformational changes in the extracellular domain of ABCA1 are associated with apoA-I binding. (2012)
  • Author(s): Nagao, K., Takahashi, K., Azuma, Y., Takada, M., Kimura, Y., Matsuo, M., Kioka, N. and Ueda, K.
  • Journal Title: J Lipid Res Volume: 53 Issue: 1 Pages: 126-136
  • DOI: 10.1194/jlr.m019976
  • Peer Reviewed
• [Presentation] Phosphorylation by protein kinase C stabilizes ABCG1 (2016)
  • Author(s): Michinori Matsuo, Taro Watanabe, and Kazumitsu Ueda
  • Organizer: 6th FEBS special meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases”
  • Place of Presentation: インスブルク（オーストリア）
  • Year and Date: 2016-03-05
  • Int'l Joint Research
• [Presentation] 多価不飽和脂肪酸を含有するグリア細胞由来リポタンパク質の神経突起伸長作用 (2015)
  • Author(s): 松尾道憲、中塔充宏、植田和光
  • Organizer: BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 多価不飽和脂肪酸含有リポタンパク質は神経突起伸長を促進する (2015)
  • Author(s): 松尾道憲、中塔充宏、植田和光
  • Organizer: 第10回トランスポーター研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-21
• [Presentation] Polyunsaturated fatty acids in lipoproteins stimulate neurite outgrowth of hippocampal neurons (2015)
  • Author(s): Michinori Matsuo, Mitsuhiro Nakato, and Kazumitsu Ueda
  • Organizer: 12th Asian Congress of Nutrition (12th ACN)
  • Place of Presentation: 横浜
  • Year and Date: 2015-05-15
  • Int'l Joint Research
• [Presentation] ABCトランスポーターの膜脂質ラフトへの分布とアミロイド前駆体タンパク質のプロセシングに与える効果 (2014)
  • Author(s): 松尾 道憲、佐野修、伊藤志保、加藤玲子、清水裕二、植田和光
  • Organizer: 第36回 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 徳島大学（徳島市）
  • Year and Date: 2014-11-21
  • Invited
• [Presentation] コレステロールを輸送するABCG1の新規機能 (2014)
  • Author(s): 松尾 道憲、渡邊太郎、平井絢子、植田 和光
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都国際会館（京都市）
  • Year and Date: 2014-10-17
  • Invited
• [Presentation] プロテインキナーゼCによるABCG1の調節 (2014)
  • Author(s): 松尾道憲、渡邊太郎、植田和光
  • Organizer: 第9回トランスポーター研究会年会
  • Place of Presentation: 名古屋市立大学（名古屋市）
  • Year and Date: 2014-06-14
• [Presentation] オメガ3脂肪酸によるリポタンパク質の質的制御を介した生理作用 (2014)
  • Author(s): 松尾 道憲、斉藤 貴将、中塔 充宏、木岡 紀幸、小川 順、植田 和光
  • Organizer: 農芸化学会2014年度大会
  • Place of Presentation: 東京
  • Invited
• [Presentation] Regulation of HMG-CoA reductase activity by ABCG1 (2014)
  • Author(s): Michinori Matsuo, Taro Watanabe, Ayako Hirai, and Kazumitsu Ueda
  • Organizer: 5th FEBS special meeting
  • Place of Presentation: Innsbruck, Austria
• [Presentation] プロテインキナーゼCによるABCG1のリン酸化解析 (2013)
  • Author(s): 松尾道憲、渡邊太郎、植田和光
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] ABC48－ヒトABCトランスポーター発現系ライブラリーの構築と細胞内局在の解析― (2013)
  • Author(s): 松尾道憲、福田麻菜、植田和光
  • Organizer: 第8回トランスポーター研究会年会
  • Place of Presentation: 熊本
• [Presentation] Phosphorylation by protein kinase C stabilizes ABCG1 (2013)
  • Author(s): Michinori Matsuo, Taro Watanabe, Kazumitsu Ueda
  • Organizer: The Fourteenth International Membrane Research Forum
  • Place of Presentation: Kyoto
• [Presentation] ABC proteins regulate the integrity of membrane meso-domains (2012)
  • Author(s): Michinori Matsuo, Hiromune Ando, Makoto Kiso, and Kazumisu Ueda
  • Organizer: iCeMS retreat
  • Place of Presentation: Osaka
• [Book] トランスポーターと疾患研究の最前線 (2013)
  • Author(s): 松尾道憲
  • Total Pages: 160
  • Publisher: 医歯薬出版
• [Remarks] 細胞生化学研究室
  • URL: http://cseika-1.kais.kyoto-u.ac.jp/