Roles of auxin in the vegetative propagation of land plants

• Project/Area Number: 24580140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kobe University (2013-2014); Kyoto University (2012)
• Principal Investigator: ISHIZAKI Kimitsune 神戸大学, 理学(系)研究科(研究院), 准教授 (00452293)
• Co-Investigator(Renkei-kenkyūsha): KOHCHI Takayuki 京都大学, 大学院生命科学研究科, 教授 (40202056) ; NISHIHAMA Ryuichi 京都大学, 大学院生命科学研究科, 講師 (70283455) ; YAMATO T. KATSUYUKI 近畿大学, 生物理工学部, 准教授 (50293915)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: オーキシン / 栄養繁殖 / 進化 / コケ植物 / 形態形成 / 陸上植物 / 転写制御 / 細胞分化 / 発生制御 / 植物ホルモン / 分裂組織 / 無性生殖 / クローン

Outline of Final Research Achievements

Vegetative propagation is a form of asexual reproduction in plants, in which new individuals with functional meristems arise without fertilization and develop directly from vegetative tissues, such as leaves, stems, and roots. The liverwort, Marchantia polymorpha produces gemmae on their gametophytes as a means of vegetative propagation. Each gemma is originated from a single epidermal cell at the bottomof the gemma-cup, developed into a mature gemma with two meristems at symmetrical position. In this study, we focused on auxin in the process of vegetative propagation. We demonstrated that machinaries of auxin signaling and biosynthesis in M. polymorpha was more or less the same as that in angiosperms. The auxin biosynthesis and responses was significantly up-regulated in the base of gemma-cup. Molecular genetic studies indicated the critical role of auxin in the both development and dormancy of gemma in M. polymorpha.

Research Products

• [Journal Article] Stomatal guard cells co-optedan ancient ABA-dependent desiccation survival system to regulate stomatal closure (2015)
  • Author(s): Lind, C., Dreyer, I., López-Sanjurjo, E.J., von Meyer, K., Ishizaki, K., Kohchi, T., Lang, D., Zhao, Y., Kreuzer, I., Al-Rasheid, K.A.S., Ronne, H., Reski, R., Zhu, J.K., Geiger, D. and Hedrich, R.
  • Journal Title: Current Biology Volume: 30 Issue: 7 Pages: 928-935
  • DOI: 10.1016/j.cub.2015.01.067
  • Peer Reviewed / Open Access
• [Journal Article] Arachidonic acid-dependent carbon-eight volatile synthesis from wounded liverwort (Marchantia polymorpha) (2015)
  • Author(s): Kihara, H., Tanaka, M., Yamato, K.T., Horibara, A., Yamada, A., Kita, S., Ishizaki, K., Kajikawa, M., Fukuzawa, H., Kohchi, T., Akakabe, Y. and Matsui, K.
  • Journal Title: Phytochemistry Volume: 107 Pages: 42-49
  • DOI: 10.1016/j.phytochem.2014.08.008
  • Peer Reviewed / Open Access
• [Journal Article] Auxin-mediated transcriptional system with a minimal set of components is critical for morphogenesis through the life cycle in Marchantia polymorpha (2015)
  • Author(s): Kato, H., Ishizaki, K., Kouno, M., Shirakawa, M., Bowman, J.L., Nishihama, R., and Kohchi, T.
  • Journal Title: PLOS Genet. Volume: In press Issue: 5 Pages: e1005084-e1005084
  • DOI: 10.1371/journal.pgen.1005084
  • NAID: 120005672595
  • Peer Reviewed / Open Access
• [Journal Article] Auxin produced by the IPyA pathway regulates development and gemmae dormancy in the 2 liverwort Marchantia polymorpha (2015)
  • Author(s): Eklund, M.D., Ishizaki, K., Flores-Sandoval, E., Kikuchi, S., Takebayashi, Y., Tsukamoto, S., Hirakawa, S., Nonomura, M., Kato, H., Kouno, M., Bhalerao, R.P., Lagercrantz, U., Kasahara, H., Kohchi, T. and Boaman, J.L.
  • Journal Title: Plant Cell Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Phytochrome-mediated regulation of cell division and growth during regeneration and sporeling development in the liverwort Marchantia polymorpha (2015)
  • Author(s): Nishihama, R., Ishizaki, K., Hosaka, M., Matsuda, Y., Kubota, A., and Kohchi, T.
  • Journal Title: J. Plant Res. Volume: 128 Issue: 3 Pages: 407-421
  • DOI: 10.1007/s10265-015-0724-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Phototropin encoded by a single-copy gene mediates chloroplast photorelocation movements in the liverwort Marchantia polymorpha (2014)
  • Author(s): Komatsu, A., Terai, M., Ishizaki, K., Suetsugu, N., Tsuboi, H., Nishihama, R., Yamato, K.T., Wada, M., and Kohchi, T.
  • Journal Title: Plant Physiol. Volume: 166 Issue: 1 Pages: 411-427
  • DOI: 10.1104/pp.114.245100
  • Peer Reviewed / Open Access
• [Journal Article] Co-option of a photoperiodic growth-phase transition system during land plant evolution. (2014)
  • Author(s): Akane Kubota, Shogo Kita, Kimitsune Ishizaki, Ryuichi Nishihama, Katsuyuki T. Yamato, Takayuki Kohchi
  • Journal Title: Nature Communications Volume: 5 : 3668 Issue: 1 Pages: 3668-3668
  • DOI: 10.1038/ncomms4668
  • Peer Reviewed
• [Journal Article] Comparison of the MpEF1α and CaMV35 promoters for application in Marchantia polymorpha overexpression studies (2014)
  • Author(s): Althoff, F., Kopischke, S., Zobell, O., Ide, K., Ishizaki, K., Kohchi, T. and Zachgo, S.
  • Journal Title: Transgenic Research Volume: 23 Issue: 2 Pages: 235-244
  • DOI: 10.1007/s11248-013-9746-z
  • Peer Reviewed / Open Access
• [Journal Article] Essential role of the E3 ubiquitin ligase NOPPERABO1 in schizogenous intercellular space formation in the liverwort Marchantia polymorpha. (2013)
  • Author(s): Ishizaki, K., Mizutani, M., Shimamura, M., Masuda, A., Nishihama, R. and Kohchi, T.
  • Journal Title: Plant Cell Volume: 25 Pages: 4075-4084
  • Peer Reviewed
• [Journal Article] Subfunctionalization of sigma factors during the evolution of land plants based on mutant analysis of liverwort (Marchantia polymorpha L.) MpSIG1. (2013)
  • Author(s): Ueda, M., Takami, T., Peng, L., Ishizaki, K., Kohchi, T., Shikanai, T. and Nishimura, Y.
  • Journal Title: Genome Biology and Evolution Volume: 5 Issue: 10 Pages: 1836-1848
  • DOI: 10.1093/gbe/evt137
  • NAID: 120005373074
  • Peer Reviewed
• [Journal Article] Characterization of four nuclear-encoded plastid RNA polymerase sigma factor genes in the liverwort Marchantia polymorpha: blue-light- and multiple stress-responsive SIG5 was acquired early in the emergence of terrestrial plants. (2013)
  • Author(s): Kanazawa T, Ishizaki K, Kohchi T, Hanaoka M, Tanaka K.
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 10 Pages: 1736-1748
  • DOI: 10.1093/pcp/pct119
  • Peer Reviewed
• [Journal Article] Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L. (2013)
  • Author(s): K. Ishizaki
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 1532-1532
  • DOI: 10.1038/srep01532
  • Peer Reviewed
• [Journal Article] Cold-induced organelle relocation in the liverwort Marchantia polymorpha L. (2013)
  • Author(s): Ogasawara Y, Ishizaki K, Kohchi T & Kodama Y
  • Journal Title: Plant, Cell & Environment Volume: 印刷中 Issue: 8 Pages: 1-9
  • DOI: 10.1111/pce.12085
  • Peer Reviewed
• [Journal Article] Efficient <i>Agrobacterium</i>-Mediated Transformation of the Liverwort <i>Marchantia polymorpha</i> Using Regenerating Thalli (2013)
  • Author(s): Kubota, A., Ishizaki, K., Hosaka, M., Kohchi T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 1 Pages: 167-172
  • DOI: 10.1271/bbb.120700
  • NAID: 10031164707
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] 基部陸上植物の光応答戦略ーフィトクロムを介した光形態形成の分子機構ー (2013)
  • Author(s): 石崎公庸、井上佳祐、河内孝之
  • Journal Title: 植物科学の最前線 (BSJ-Review) Volume: 4 Pages: 33-44
• [Journal Article] Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha (2012)
  • Author(s): Ueda M., Kuniyoshi T., Yamamoto Y., Sugimoto K., Ishizaki K., Kohchi T., Nishimura Y. and Shikanai T
  • Journal Title: Plant J. Volume: 72 Issue: 4 Pages: 683-693
  • DOI: 10.1111/j.1365-313x.2012.05115.x
  • Peer Reviewed
• [Journal Article] Characterization of the plasma membrane H+-ATPase in the liverwort Marchantia polymorpha. (2012)
  • Author(s): Okumura M, Inoue S, Takahashi K, Ishizaki K, Kohchi T, Kinoshita T.
  • Journal Title: Plant Physiology Volume: 159 Issue: 2 Pages: 826-834
  • DOI: 10.1104/pp.112.195537
  • Peer Reviewed
• [Journal Article] Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha (2012)
  • Author(s): Ishizaki K., Nonomura M., Kato H., Yamato K.T., Kohchi T.
  • Journal Title: Journal of Plant Research Volume: 125 Issue: 5 Pages: 826-834
  • DOI: 10.1007/s10265-012-0477-7
  • Peer Reviewed
• [Journal Article] 古くて新しいモデル植物としてのタイ類ゼニゴケの特徴 (2012)
  • Author(s): 河内孝之、石崎公庸
  • Journal Title: 植物科学の最前線 (BSJ-Review) Volume: 3 Pages: 58-70
• [Journal Article] 生物材料インデックス「ゼニゴケ」 (2012)
  • Author(s): 石崎公庸、河内孝之
  • Journal Title: 生物工学会誌 Volume: 90 Pages: 600-603
• [Presentation] Vegetative propagation: development of asexual progenies from vegetative tissue (2015)
  • Author(s): Kimitsune Ishizaki
  • Organizer: 第56回日本植物生理学会年会 シンポジウム
  • Place of Presentation: 東京農業大学・世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
  • Invited
• [Presentation] R2R3－MYB型転写因子GCAM1は苔類ゼニゴケの杯状体形成を制御する (2015)
  • Author(s): 塚本成幸、菅谷友美、大和勝幸、西浜竜一、久保浩義、七條千津子、深城英弘、三村徹郎、河内孝之、石崎公庸
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学・世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 無性芽形成異常変異体karappo2における変異原因遺伝子の同定 (2015)
  • Author(s): 樋渡琢真、山口勝司、重信秀治、澤進一郎、深城英弘、三村徹郎、河内孝之、石崎公庸
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学・世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ゼニゴケにおける杯状体形成関連遺伝子GEMMA-CUP ASSOCIATED MYB 2の解析 (2015)
  • Author(s): 高見英幸、塚本成幸、増田晃秀、七條千津子、深城英弘、三村徹郎、河内孝之
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学・世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ゼニゴケから見えてきた栄養繁殖と腋芽発生の共通制御メカニズム (2014)
  • Author(s): 石崎公庸
  • Organizer: 日本植物学会第78回大会 シンポジウム
  • Place of Presentation: 明治大学・生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
  • Invited
• [Presentation] ゼニゴケの栄養繁殖器官で発現するR2R3型MYB遺伝子の単離と機能解析 (2014)
  • Author(s): 塚本成幸、菅谷友美、大和勝幸、山口勝司、重信秀治、西浜竜一、七條千津子、久保浩義、河内孝之、深城英弘、三村徹郎、石崎公庸
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学・生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] コケ植物配偶体における器官発生の分子遺伝学 (2014)
  • Author(s): 石崎公庸
  • Organizer: イネ遺伝学・分子生物学ワークショップ2014
  • Place of Presentation: 東京大学弥生講堂一条ホール
  • Year and Date: 2014-07-11 – 2014-07-12
  • Invited
• [Presentation] ゼニゴケから探るオーキシン応答の基本メカニズムとその進化 (2013)
  • Author(s): 石崎公庸、加藤大貴、河内孝之
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Invited
• [Presentation] 基部陸上植物ゼニゴケにおける概日時計の分子ネットワーク (2013)
  • Author(s): 久保田茜、喜多祥吾、村中智昭、石崎公庸、大和 勝幸、青木摂之、小山時隆、河内孝之
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] 苔類ゼニゴケにおけるGI, FKFを介した日長依存的な成長制御機構 (2013)
  • Author(s): 喜多祥吾、久保田茜、石崎公庸、西浜竜一、河内 孝之
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] ゼニゴケの赤色光によるサイクリンD遺伝子発現制御機構 (2013)
  • Author(s): 西浜竜一、眞鍋諒、中村衣里、井上佳祐、石崎公 庸、河内孝之
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] ゼニゴケで探るコケ植物の光応答と発生制御 (2012)
  • Author(s): 石崎公庸、河内孝之
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
  • Invited
• [Presentation] ゼニゴケのALTERED MERISTEM PROGRAM 1 (AMP1) 変異体はオーキシン低感受性を示す (2012)
  • Author(s): 桐田啓如、武田真由子、石崎公庸、河内孝之
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
• [Presentation] ゼニゴケ気室形成制御遺伝子NOPPERABO1の単離と解析 (2012)
  • Author(s): 水谷未耶，石崎公庸，増田晃秀，桐田啓如，西浜 竜一，河内孝之
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
• [Presentation] Forward genetics for gametophyte development in Marchantia polymorpha (2012)
  • Author(s): Ishizaki, K., Mizutani, M., Masuda, A., Shimamura, M., Nishihama, R. and Kohchi, T.
  • Organizer: International Marchantia Workshop 2012
  • Place of Presentation: ホテルグリーンピア南阿蘇
• [Presentation] コケ植物における無性芽の発生と休眠
  • Author(s): 石崎公庸
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス
  • Invited
• [Presentation] ゼニゴケ研究地平への投射：オーキシン信号伝達を例に
  • Author(s): 河内孝之、加藤大貴、西浜竜一、大和勝幸、石崎公庸
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス
  • Invited
• [Presentation] 転写因子の相互作用によるゼニゴケのオーキシン信号伝達ネットワーク
  • Author(s): 加藤大貴、神埜勝、白川一、石崎公庸、西浜竜一、河内孝之
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス
• [Remarks] 神戸大学ー石崎研究室ホームページ
  • URL: http://www.research.kobe-u.ac.jp/fsci-ishizaki/